stigmasterol and beta-amyrin

To study the chemical constituents of Suaeda glauca.. The chemical constituents were isolated and purified with several separation and purification techniques. Their structures were identified by physicochemical properties and various spectroscopic methods.. Ten compounds were isolated from the ethyl acetate fraction as lignoceric acid (1), β-amyrin-n-nonyl ether(2), β-sitosterol(3), β-daucosterol(4), quercetin(5), luteolin(6), luteolin-7-O-β-D-glucoside(7), isorhamnetin(8), scopoletin (9) and stigmasterol(10).. Compounds 1, 2, 6, 7, 8, 9 and 10 are isolated from Suaeda genus for the first time and compounds 3 - 5 are isolated from this plant for the first time.

Topics: Acetates; Chenopodiaceae; Glucosides; Luteolin; Oleanolic Acid; Phytochemicals; Plant Extracts; Quercetin; Sitosterols; Stigmasterol

To study the chemical constituents from Hibiscus syriacus leaves and their α-glucosidase inhibitory activities.. Column chromatography including macroporous resins, silica gel and Sephadex LH-20 were used for the isolation and purification of all compounds. Spectroscopic methods including physical and chemical properties, 1H-NMR and 13C-NMR were used for the identification of structures. Their α-glucosidase inhibitory activities were detected by a 96-well microplate.. 15 compounds were isolated and identified as β-sitosterol(1), β-daucostero (2), β-amyrin (3), oleanolic acid (4), stigmast-4-en-3-one (5), friedelin (6), syriacusin A (7), kaempferol (8), isovitexin (9), vitexin (10), apigenin (11), apigenin-7-O-β-D-glucopyranoside (12), luteolin-7-O-β-D-glucopyranoside (13), vitexin-7-O-β-D-glucopyranoside (14) and rutin (15).. All the compounds are isolated from the leaves of Hibiscus syriacus for the first time. Taking acarbose as positive control, the α-glucosidase inhibitory activities of 15 compounds were evaluated. Compounds 7 and 9 have shown strong α-glucosidase inhibitory activities with IC50 of 39.03 ± 0.38 and 32.12 ± 0.62 mg/L, inhibition ratio of 94.95% and 97.15%, respectively.

Topics: alpha-Glucosidases; Apigenin; Glucosides; Glycoside Hydrolase Inhibitors; Hibiscus; Kaempferols; Luteolin; Oleanolic Acid; Phytochemicals; Plant Leaves; Sitosterols; Stigmasterol; Triterpenes

To study the chemical constituents from Lysimachia paridiformis.. The column chromatographic techniques were applied to isolate the chemical constituents of Lysimachia paridiformis. EI-MS and NMR were used to identify the structures.. Seven compounds were isolated and identified from the extract of L. paridiformis, and their structures were elucidated as stigmasterol (1), oleanolic aicd (2), beta-amyrin (3), quercetin (4), luteolin (5), quercetin-3-O-beta-D-galactoside (6) and beta-daucosterol (7).. Compound 1 -7 are isolated from this plant for the first time.

Topics: Luteolin; Magnetic Resonance Spectroscopy; Oleanolic Acid; Primulaceae; Quercetin; Stigmasterol

The Lecythidaceae family is composed of 25 genera and 400 species that occur in the form of trees with a pantropical distribution. The genera Cariniana belongs to the family Lecythidaceae, and there are few reports considering these species. In this work, fractionation of the dichloromethane fraction obtained from the aqueous extract of the stem bark of Cariniana domestica (Mart) Miers, popularly known as Jequitibá-roxo, led to the isolation of two mixtures of triterpenoids: lupeol and β-amyrin and β-sitosterol and stigmasterol. The structures of the isolated compounds were elucidated by spectroscopic (NMR) and chromatographic (GC-MS) techniques as well as literature data comparisons. β-Sitosterol, stigmasterol, lupeol and β-amyrin were quantified in dichloromethane fraction by high-performance liquid chromatography (HPLC/DAD). The dichloromethane fraction was also investigated for antioxidant and antifungal activities. The isolated compounds and their biological activities are reported for the first time for the species C. domestica.

Topics: Antifungal Agents; Antioxidants; Chromatography, High Pressure Liquid; Gas Chromatography-Mass Spectrometry; Lecythidaceae; Magnetic Resonance Spectroscopy; Methylene Chloride; Molecular Structure; Oleanolic Acid; Pentacyclic Triterpenes; Plant Bark; Plant Extracts; Saccharomyces cerevisiae; Sitosterols; Stigmasterol; Triterpenes

Jatropha tanjorensis was investigated scientifically to generate evidence for the efficacies reported in traditional systems and the results are given here.. Different concentrations of the solvent extracts of leaves and four isolated compounds were tested against human pathogenic microorganisms such as gram-positive bacteria of Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermis, gram-negative bacteria of Aeromonas hydrophila, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Proteus vulgaris, Salmonella paratyphi, Salmonella paratyphi A, Vibrio alcaligenes, Vibrio cholerae and fungi of Aspergillus fumigatus, Candida albicans, Microsporum gypseum and Trichophyton rubrum by agar-well diffusion and disk diffusion methods.. In agar-well diffusion method, maximum activity was recorded in a concentration-dependent manner. The extracts recorded activity against bacteria such as 17-26, 15-25 and 13-24 mm to methanol extract and 15-24, 14-23 and 12-22 mm to chloroform extract at 50, 25 and 12.5 mg/ml respectively and fungi such as 9-15 mm to A. fumigatus and 5-16 mm to T. rubrum. Maximum activity was 30-46, 27-43 and 17-40 mm to friedelin and 23-46, 28-44 and 18-41 mm to R (+) 4-hydroxy-2-pyrrolidinone against bacteria and 12-37, 8-34 and 31-33 mm to friedelin and 12-40, 11-35 and 10-33 mm to R (+) 4-hydroxy-2-pyrrolidinone against fungi at 10, 5 and 2.5mg respectively.. The present study concludes that friedelin, β-amyrin, stigmasterol and R (+) 4-hydroxy-2-pyrrolidinone present in the methanol extract could be responsible for the broad spectrum of antimicrobial activity and provide scientific evidence.

Topics: Anti-Bacterial Agents; Antifungal Agents; Bacteria; Fungi; Humans; Jatropha; Microbial Sensitivity Tests; Oleanolic Acid; Plant Extracts; Plant Leaves; Pyrrolidinones; Stigmasterol; Triterpenes

A new compound, 3-O-alpha-L-arabinopyranosyl-4-hydroxybenzoic acid (13), in addition to 16 newly reported compounds: alpha-amyrin acetate (1), beta-amyrone (2), 3beta-acetoxy-20-taraxasten-22-one (3), alpha-amyrin (4), ceryl alcohol (5), stigmasterol (6), beta-sitosterol (7), 2alpha,3alpha-dihydroxy-lup-20(29)-en-28-oate (8), ursolic acid (9), beta-sitosterol-3-O-glucosoide (10), protocatechuic acid (11), betulinic acid (12), quercetin (14), quercetin-3-O-beta-D-glucoside (15), kampferol-3-O-beta-neohesperidoside (16) and rutin (17) were isolated from the stem bark and leaves of Ficus pandurata (Hance) cultivated in Egypt. Identification of these compounds has been established by physical, chemical and spectral data (UV, IR, MS, (1)H- and (13)C-NMR), as well as comparison with authentic samples.

Topics: Betulinic Acid; Ficus; Magnetic Resonance Spectroscopy; Molecular Structure; Oleanolic Acid; Pentacyclic Triterpenes; Plant Bark; Plant Leaves; Sitosterols; Spectrophotometry, Infrared; Spectrophotometry, Ultraviolet; Stigmasterol; Triterpenes; Ursolic Acid

To study the chemical constituents from the roots of Ficus hirta.. Compounds were isolated by chromatographic techniques on silica gel and HP-20 resin columns. Their structures were elucidated by chemical and spectroscopic methods.. Ten compounds were identified as beta-sitosterol (1), stigmasterol (2), psoralene (3), 3beta-hydroxy-stigmast-5-en-7-one (4), 5-hydroxy-4', 6, 7, 8-tetramethoxy flavone (5), 4', 5, 6, 7, 8-pentamethoxy flavone (6), 4', 5, 7-trihydroxy-flavone (7), 3beta-acetoxy-beta-amyrin (8), 3beta-acetoxy-alpha-amyrin (9) and hesperidin (10).. The compounds 4, 5, 6 were obtained from this genus for the first time, and all the compounds were obtained from this plant for the first time.

Topics: Ficus; Ficusin; Flavones; Oleanolic Acid; Plant Roots; Plants, Medicinal; Sitosterols; Stigmasterol

North American ginseng (Panax quinquefolium L.) oil was saponifed and the unsaponifiable matter trimethylsilylated. The phytosterol fraction of hexane-extracted, air-dried seed was quantified and identified by GC and GC-MS. Phytosterol contents (milligrams per 100 g of oil) were as follows: squalene (514-569), oxidosqualene (8.97-48.2), campesterol (9.96-12.4), stigmasterol (93.2-113), clerosterol (1.91-2.14), beta-sitosterol (153-186), beta-amyrin (11.7-19.5), delta(5)-avenasterol (12.4-20.5), delta(5,24(25))-stigmasterol (3.70-.76), lupeol (14.4-15.2), delta(7)-sitosterol (12.5-14.6), delta(7)-avenasterol (4.11-8.09), 24-methylenecycloartanol (1.94-4.76), and citrostadienol (2.50-3.81). Seed stratification lowered the phytosterol levels. Oven-drying gave mixed results, and phytosterols varied slightly between the 1999 and 2000 harvests.

Topics: Cholesterol; Chromatography, Gas; Gas Chromatography-Mass Spectrometry; Oleanolic Acid; Panax; Phytosterols; Plant Oils; Seeds; Sitosterols; Squalene; Stigmasterol; Triterpenes

Six compounds were isolated from Begonia evansiana. By means of TLC, mp, 1H and 13CNMR, they were idenified as beta-sitosterol, beta-amyrin, daucosterol, stigmasterol, stigmasterol-3-O-beta-D-glucopyranoside and 4', 5', 7-trihydroxy-flavone-6-O-beta-D-glucopyranoside. All the compounds were isolated from this plant for the first time.

Topics: Drugs, Chinese Herbal; Flavonoids; Glucosides; Magnoliopsida; Molecular Structure; Oleanolic Acid; Plants, Medicinal; Sitosterols; Stigmasterol; Triterpenes