stigmasterol and 7-hydroxycoumarin

To isolate and identify the chemical constituents of the root of Chloranthus multistachys Pei.. The compounds were isolated by column chromatography, semi-preparative thin layer chromatography and related techniques, their structures were elucidated through spectroscopic analyses.. Nine compounds were isolated and identified as: lupeol (I), cycloeucalenol (II), isofragidin (III), daphnin (IV), umbelliferone (V), palmitic acid (VI), stigmasterol (VII), beta-sitosterol (VIII), beta-daucosterol (IX).. Except VI, all compounds are isolated from this plant for the first time.

Topics: China; Coumarins; Magnoliopsida; Molecular Structure; Pentacyclic Triterpenes; Phytosterols; Plant Roots; Plants, Medicinal; Stigmasterol; Umbelliferones

The antitermite (termiticidal and antifeedant) activity of Protium javanicum Burm. f. extract was investigated. The ethyl acetate fraction was active. Scopoletin (1), quercetin, and stigmasterol were isolated by bioassay-guided fractionation. Scopoletin had the highest activity among the three compounds. In order to investigate the structure-activity relationship (SAR) of the methoxy and hydroxy groups at the C-6 and C-7 positions of the coumarin skeleton, we synthesized several coumarin derivatives whose chemical structures are similar to scopoletin. Scopoletin exhibited the strongest termiticidal activity among the 10 compounds tested, followed by 6-methoxycoumarin (3), 6-hydroxycoumarin (7), and umbelliferone (8). All compounds except coumarin (9) showed antifeedant activity.

Topics: Animals; Burseraceae; Coumarins; Insecticides; Isoptera; Plant Extracts; Quercetin; Scopoletin; Stigmasterol; Structure-Activity Relationship; Umbelliferones