stigmasterol and 4-hydroxybenzoic-acid

To study the chemical constituents from Macaranga denticulata Root.. The chemical constituents were isolated and purified by silica-gel column chromatography and recrystallization, and their structures were identified by physicochemical properties and spectral data.. Nine compounds were isolated and identified as: gheddic acid (1), aleuritolic acid-3-acetate (2), β-sitosterol (3), stigmast-4-en-6β-ol-3 -one (4), 2α-hydroxyaleuritolic acid 3-p-hydroxybenzoate (5), scopoletin (6), daucosterol (7), 2, 6-dimethoxy-1,4-benzoquinone (8) and maslinic acid(9).. Compounds 1-9 are obtained from this plant for the first time.

Topics: Benzoquinones; Euphorbiaceae; Parabens; Phytochemicals; Plant Roots; Plants, Medicinal; Scopoletin; Sitosterols; Stigmasterol; Triterpenes

To investigate the chemical constituents in water-soluble fraction of Carthamus tinctorius.. Compounds were isolated and purified by macroporus resin, silica gel, Sephadex LH-20 column chromatography and preparative HPLC. The structures were identified by spectral analysis.. Twelve compounds were isolated and identified as 4-Hydroxybenzaldehyde (1); E-1-(4'-hydroxypheny) -but-1-en-3-one (2); 3-Formylindole (3); 2-Acetyl-5-hydroxymethylfuran (4); p-Hydroxycinnamic acid (5); (6R, 7E, 9R) -9-hydroxy-4,7-megastigmandien-3-one (6); 4-hydroxyacetophenone (7); 5-(hydroxymethyl) -2-furaldehyde (8); 4-Hydroxybenzoic acid (9); Stigmasterol-3-O-beta-D-glucopyranoside (10); Daucosterol (11); beta-sitosterol (12).. Compounds 1 - 4, 6, 7, 10 are isolated from this plant for the first time.

Topics: Acetophenones; Benzaldehydes; Carthamus tinctorius; Chromatography, High Pressure Liquid; Coumaric Acids; Flowers; Furaldehyde; Glucosides; Indoles; Molecular Structure; Parabens; Plants, Medicinal; Propionates; Stigmasterol

Two new diterpenoids, 14,18-dihydroxyabieta-8,11,13-trien-7-one (1) and 13-acetyl-14,18-dihydroxy-podocarpa-8,11,13-triene (2), together with eight known compounds, i.e., gaultheric acid (3), vanillic acid (4), 4-hydroxybenzoic acid (5), cinnamic acid (6), stearic acid (7), palmitic acid (8), beta-sitosterol (9), and stigmasterol (10), were isolated from the MeOH extract of the whole plant of Gaultheria itoana Hayata (Ericaceae). The structures of the new constituents were elucidated by spectroscopic methods (UV, IR, and 1D- and 2D-NMR) and by mass spectrometry (HR-ESI-MS). Among them, 1 and 2 were demonstrated to exhibit significant cytotoxic activity against the LNCaP cell line.

Topics: Antineoplastic Agents, Phytogenic; Cell Line, Tumor; Cinnamates; Diterpenes; Gaultheria; Humans; Lung Neoplasms; Magnetic Resonance Spectroscopy; Methanol; Molecular Structure; Palmitic Acid; Parabens; Plant Extracts; Sitosterols; Spectrometry, Mass, Electrospray Ionization; Stearic Acids; Stigmasterol; Vanillic Acid

To study the chemical constituents of Dendobium crystallinum.. Compounds were isolated and purified by silica gel and Sephadex LH-20 column chromatography. Their structures were identified by physicochemical properties and spectral analyses.. Nine compounds were obtained and identified as: 4, 4'-dihydroxy-3, 5-dimethoxybi-benzyl (1), gigantol (2), naringenin (3) , p-hydroxybenzoic acid (4), n-tetracosyl trans-p-cou-marate (5), n-octacosy trans-p-coumarate (6), n-hexacosyl trans-ferulate (7), stigmasterol (8), daucosterol (9).. All these compounds were obtained from this plant for the first time, compounds 1 and 4 were isolated firstly from the genus.

Topics: Bibenzyls; Chromatography; Dendrobium; Drugs, Chinese Herbal; Flavanones; Guaiacol; Magnetic Resonance Spectroscopy; Parabens; Sitosterols; Stigmasterol

To investigate the chemical constituents of the brown alga D. divaricata, and to test cytotoxicities of the purified compounds.. Compounds were isolated by normal phase silica gel, Sephadex LH-20 chromatography and reverse phase HPLC techniques. Their structures were elucidated by spectroscopic methods including IR, MS and NMR. Cytotoxicities were tested by MTT method.. Eight compounds were isolated from ethanolic extract of the brown alga D. divaricata and their structures were identified as (-)-torreyol (I), 4beta, 5alpha-dihydroxycubenol (II), 3-farnesyl-p-hydroxybenzioc acid (III), chromazonarol (IV), fucosterol (V), phenyl acetylamine (VI), 4-hydroxybenzoic acid (VII) and n-hexadecanoic acid (VIII).. Compound II and IV were obtained from this alga for the first time. The others were isolated from the Dictyotaceae algae for the first time. All compounds were inactive (IC50 > 10 microg x mL(-1)) against human tumor cell lines KB, Bel-7402, PC-3M, Ketr 3 and MCF-7.

Topics: Cell Line, Tumor; Humans; Parabens; Phaeophyceae; Stigmasterol; Terpenes; Xanthenes