stigmasterol and 10-nonacosanol

Preparative high-speed counter-current chromatography (HSCCC), as a continuous liquid-liquid partition chromatography with no solid support matrix, combined with evaporative light scattering detection (ELSD) was employed for systematic separation and purification of non-chromophoric chemical components from Chinese medicinal herb Adenophora tetraphlla (Thunb.), Fisch. Nine compounds, including alpha-spinasterol, beta-sitosterol, nonacosan-10-ol, 24-methylene cycloartanol, lupenone, 3-O-palmitoyl-beta-sitosterol, 3-O-beta-d-glucose-beta-sitosterol, eicosanoic acid and an unknown compound, were obtained. The compounds were all above 95% determined by high-performance liquid chromatography (HPLC)-ELSD, and their structures were identified by (1)H NMR and chemical ionization mass spectroscopy (CI-MS). The results demonstrate that HSCCC coupled with ELSD is a feasible and efficient technique for systematic isolation of non-chromophoric components from traditional medicinal herbs.

Topics: Campanulaceae; Chromatography, High Pressure Liquid; Countercurrent Distribution; Light; Phytosterols; Plant Roots; Sitosterols; Stigmasterol; Triterpenes

To search for the effective anti-epilepsiy components from the Chinese folk drug Langdu, the root of Euphorbia ebracteolata.. Chromatography and spectroscopic analysis were used to isolate and elucidate the chemical constituents in the plant.. Four compounds, namely 10-nonacosanol, 24-methylenecycloartanol, stigmasterol and dacosterol have been isolated from the precipitate of the plant's methanol extract.. 10-nonacosarnol and 24-methylenecycloartanol were obtained from the root of E. ebracteolate for the first time.

Topics: Euphorbia; Plant Roots; Plants, Medicinal; Stigmasterol; Triterpenes