stearates and parinaric-acid

The effects of sterol carrier protein-2 (SCP-2) expression on fatty acid uptake and cytoplasmic diffusion were determined using L cell fibroblasts transfected with cDNA encoding either the 15- or 13. 2-kDa form of SCP-2. Cis-parinarate and 12-N-methyl-(7-nitrobenz-2-oxa-1,3-diazol)aminostearate (NBD-stearate) were used as nonesterifiable fluorescent fatty acid probes. NBD-stearate and cis-parinarate uptake was rapid and saturable. In 15-kDa SCP-2-expressing cells, the extent of NBD-stearate and cis-parinarate uptake was increased 1.4- and 1. 2-fold, respectively, compared with control. In the 13.2-kDa SCP-2-expressing cells, the extent of NBD-stearate and cis-parinarate uptake was increased 1.3- and 1.1-fold, respectively, compared with control cells. NBD-stearate cytoplasmic diffusion was increased 1.5-fold in 15-kDa SCP-2-expressing cells, but not in 13. 2-kDa SCP-2-expressing cells, compared with control cells. After incubation with NBD-stearate for 30 min at 37 degrees C, fluorescence imaging indicated that NBD-stearate was localized primarily in lipid droplets in all cell lines. These results suggest that SCP-2 may be involved not only in fatty acid uptake but also in intracellular fatty acid trafficking.

Topics: 4-Chloro-7-nitrobenzofurazan; Analysis of Variance; Animals; Biological Transport; Carrier Proteins; Cytoplasm; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Fatty Acids, Unsaturated; Kinetics; L Cells; Mice; Microscopy, Fluorescence; Molecular Weight; Myelin P2 Protein; Neoplasm Proteins; Nerve Tissue Proteins; Plant Proteins; Recombinant Proteins; Stearates; Sterols; Transfection

Topics: Allosteric Regulation; Allosteric Site; Electron Spin Resonance Spectroscopy; Fatty Acids, Unsaturated; Humans; Kinetics; Protein Binding; Serum Albumin; Spin Labels; Stearates; Stearic Acids