stearates and methyl-palmitate

Banana plants (

Topics: Animals; Antinematodal Agents; Caenorhabditis elegans; Gas Chromatography-Mass Spectrometry; Musa; Palmitates; Plant Diseases; Plant Roots; Stearates; Tylenchoidea

Two methyl esters of fatty acids, namely octadecanoic acid methyl ester (methyl stearate) and hexadecanoic acid methyl ester (methyl palmitate), in addition to four cinnamyl alcohol derivatives, sinapyl alcohol, coniferyl alcohol, p-coumaryl alcohol and coniferyl alcohol 4-O-glucoside (coniferin), were isolated from callus cultures of Wedelia prostrata. The structure of coniferin was established by spectroscopic and chemical methods, while the other compounds were identified by gas chromatography-mass spectrometry and thin layer chromatography in comparison with standards.

Topics: Cell Culture Techniques; Chromatography, Thin Layer; Cinnamates; Coumaric Acids; Esters; Gas Chromatography-Mass Spectrometry; Methanol; Palmitates; Phenols; Phenylpropionates; Plant Shoots; Propanols; Propionates; Stearates; Wedelia

Si-wu decoction (SDE), a classic prescription in Traditional Chinese Medicine, has been used for the treatment of a variety of anaemia in China for centuries. In order to explore the scientific basis of the formula, we investigated the relationship between its chemical components and proliferation-promoting effects on rat marrow-derived mesenchymal stem cells (MSCs). Twenty (F-1-F-20) components were obtained and their proliferation-promoting effects on MSCs were investigated. The results showed that F-4, F-7, F-10, and F-11 stimulated the proliferation of the MSCs. The chemical components with proliferation-promoting effects on the MSCs were further identified by GC-MS, HPLC, LC-MS, and other spectra. Ligustilide (F-4) isolated from SDE showed the best proliferation-promoting effect. Palmitic acid methyl ester and stearic acid ethyl identified from F-7 and F-10 by HPLC were also confirmed to be responsible for stimulating MSC proliferation. A novel compound, 6,7-dihydroxy-3-(-prop-1-enyl)isobenzofuran-1(3H)-one, was found in the SDE for the first time by LC-MS(n), whose structure was similar to ligustilide.

Topics: 4-Butyrolactone; Animals; Benzofurans; Cell Proliferation; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; Flow Cytometry; Gas Chromatography-Mass Spectrometry; Mesenchymal Stem Cells; Molecular Structure; Palmitates; Rats; Rats, Sprague-Dawley; Stearates; Structure-Activity Relationship

A new infrared spectroscopic method suitable for determining total fatty alcohol and fatty acid ester concentrations in industrial oils has been developed. Oil samples were diluted with toluene (1:3 w/w), the toxicity and volatility of which are relatively low compared with more commonly used IR solvents, like carbon tetrachloride or carbon disulfide. Mixture standards were prepared from dodecanol, tetradecanol, octadecanol, methyl stearate and methyl palmitate. Some analytical and statistical tests were performed on the developed method. The recoveries and the repeatability of the method proved to be sufficient for the quantitative determination of fatty alcohol and fatty acid ester additives in industrial oils. Reproducibility testing in another laboratory also produced satisfactory results. The developed method also proved to be relatively quick and simple. This method was developed to satisfy industry's need to determine the concentrations of these oil additives, and it has already been applied successfully in machinery oil analysis.

Topics: Carbon Disulfide; Carbon Tetrachloride; Dodecanol; Esters; Fatty Acids; Fatty Alcohols; Palmitates; Plant Oils; Solvents; Spectrophotometry, Infrared; Stearates; Volatilization