stearates has been researched along with ethyl-oleate* in 11 studies
11 other study(ies) available for stearates and ethyl-oleate
Article | Year |
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Investigation of Structural Features of Two Related Lipases and the Impact on Fatty Acid Specificity in Vegetable Fats.
One of the indispensable applications of lipases in modification of oils and fats is the possibility to tailor the fatty acid content of triacylglycerols (TAGs), to meet specific requirements from various applications in food, nutrition, and cosmetic industries. Oleic acid (C18:1) and stearic acid (C18:0) are two common long fatty acids in the side chain of triglycerides in plant fats and oils that have similar chemical composition and structures, except for an unsaturated bond between C9 and C10 in oleic acid. Two lipases from Topics: Fungal Proteins; Lipase; Molecular Docking Simulation; Oleic Acids; Rhizomucor; Rhizopus oryzae; Sequence Analysis, Protein; Stearates; Structure-Activity Relationship; Substrate Specificity | 2022 |
Determination of ethyl glucuronide and fatty acid ethyl esters in hair samples.
Hair testing for alcohol biomarkers is an important tool for monitoring alcohol consumption. We propose two methods for assessing alcohol exposure through combined analysis of ethyl glucuronide (EtG) and fatty acid ethyl esters (FAEEs) species (ethyl myristate, palmitate, stearate and oleate) in hair (30 mg). EtG was analysed by liquid chromatography-tandem mass spectrometry, while FAEEs were analysed by gas chromatography-tandem mass spectrometry using electron impact ionization. Both methods were validated according to internationally accepted guidelines. Linearity was proven between 3 and 500 pg/mg for EtG and 30-5000 pg/mg for FAEEs, and the limits of quantification were 3 pg/mg for EtG and 30 pg/mg for each of the four FAEEs. Precision and accuracy were considered adequate, processed EtG samples were found to be stable for up to 96 h left in the injector and processed FAEEs samples for up to 24 h. Matrix effects were not significant. Both methods were applied to the analysis of 15 authentic samples, using the cut-off values proposed by the Society of Hair Testing for interpretation. The results agreed well with the self-reported alcohol consumption in most cases, and demonstrated the suitability of the methods to be applied in routine analysis of alcohol biomarkers, allowing monitoring consumption using low sample amounts. Topics: Adult; Alcohol Drinking; Biomarkers; Child, Preschool; Esters; Fatty Acids; Gas Chromatography-Mass Spectrometry; Glucuronates; Hair; Humans; Limit of Detection; Myristates; Oleic Acids; Palmitic Acids; Reproducibility of Results; Solid Phase Extraction; Stearates; Tandem Mass Spectrometry | 2017 |
2014 consensus for the use of alcohol markers in hair for assessment of both abstinence and chronic excessive alcohol consumption.
Topics: Alcohol Abstinence; Alcoholism; Biomarkers; Chronic Disease; Consensus; Forensic Toxicology; Glucuronates; Hair; Hair Preparations; Humans; Myristates; Oleic Acids; Palmitic Acids; Stearates; Substance Abuse Detection | 2015 |
A SPME-GC/MS procedure for the determination of fatty acid ethyl esters in hair for confirmation of abstinence test results.
Fatty acid ethyl esters (FAEE), direct metabolites of ethanol, are suitable alcohol markers that can be detected in different tissues. The determination of FAEE in hair can help to evaluate social and excessive alcohol consumption. Due to the presence of FAEE in the hair of teetotalers, proving alcohol abstinence seems to be impossible. To verify these results, an solid phase micro extraction-gas chromatography/mass spectrometry procedure for the determination of the four FAEE: ethyl myristate, ethyl palmitate, ethyl oleate and ethyl stearate in hair was validated with special focus on low concentration levels. Besides very high sensitivity (limits of detection between 0.005 and 0.009 ng/mg), good results for linearity, precision and accuracy, recovery and stability were achieved. In addition, 73 hair samples with measured ethyl glucuronide (EtG) concentrations between 4 and 10 pg/mg were analyzed for FAEE. By using the following cut-offs: EtG: 7 pg/mg, FAEE: 0.2 ng/mg a satisfying matching rate of 72.6% was found. This shows that FAEE can be determined to verify borderline EtG concentrations even in the context of abstinence tests. However, the diversified influencing factors on analyte concentrations in hair, which may explain the large deviations between EtG and FAEE results observed in some cases, have to be mentioned when interpret ambiguous results. Topics: Alcohol Drinking; Fatty Acids; Gas Chromatography-Mass Spectrometry; Glucuronates; Hair; Humans; Limit of Detection; Myristates; Oleic Acids; Palmitic Acids; Reproducibility of Results; Solid Phase Microextraction; Stearates; Substance Abuse Detection | 2014 |
Kinetic study of the prooxidant effect of alpha-tocopherol. Hydrogen abstraction from lipids by alpha-tocopheroxyl radical.
A kinetic study of the prooxidant effect of alpha-tocopherol was performed. The rates of allylic hydrogen abstraction from various unsaturated fatty acid esters (ethyl stearate 1, ethyl oleate 2, ethyl linoleate 3, ethyl linolenate 4, and ethyl arachidonate 5) by alpha-tocopheroxyl radical in toluene were determined, using a double-mixing stopped-flow spectrophotometer. The second-order rate constants (k (p)) obtained are <1 x 10(-2) M(-1 )s(-1) for 1, 1.90 x 10(-2) M(-1 )s(-1) for 2, 8.33 x 10(-2 )M(-1 )s(-1) for 3, 1.92 x 10(-1) M(-1 )s(-1) for 4, and 2.43 x 10(-1 )M(-1 )s(-1) for 5 at 25.0 degrees C. Fatty acid esters 3, 4, and 5 contain two, four, and six -CH(2)- hydrogen atoms activated by two pi-electron systems (-C=C-CH(2)-C=C-). On the other hand, fatty acid ester 2 has four -CH(2)- hydrogen atoms activated by a single pi-electron system (-CH(2)-C=C-CH(2)-). Thus, the rate constants, k (abstr)/H, given on an available hydrogen basis are k (p)/4 = 4.75 x 10(-3 )M(-1 )s(-1) for 2, k (p)/2 = 4.16 x 10(-2) M(-1 )s(-1) for 3, k (p)/4 = 4.79 x 10(-2 )M(-1 )s(-1) for 4, and k (p)/6 = 4.05 x 10(-2 )M(-1 )s(-1) for 5. The k (abstr)/H values obtained for 3, 4, and 5 are similar to each other, and are by about one order of magnitude higher than that for 2. From these results, it is suggested that the prooxidant effect of alpha-tocopherol in edible oils, fats, and low-density lipoproteins may be induced by the above hydrogen abstraction reaction. Topics: alpha-Tocopherol; Arachidonic Acids; Free Radicals; Hydrogen; Linoleic Acids; Linolenic Acids; Lipids; Oleic Acids; Oxidants; Stearates; Vitamin E | 2009 |
Controlling for serum albumin level improves the correlation between serum fatty acid ethyl esters and blood ethanol level.
Fatty acid ethyl esters (FAEEs) are generated by the nonoxidative metabolism of alcohol and correlate positively with blood alcohol levels (BAL). As FAEEs are produced predominantly in the liver and bind to albumin in plasma, blood FAEE concentrations may be affected by serum albumin levels. The aim of this exploratory study was to define the relationship of FAEE levels with BAL after adjustment for serum albumin concentration.. Fatty acid ethyl ester, BAL, and albumin concentrations were measured from de-identified, ethanol-containing serum samples (N=18). The assay focused on 3 FAEE species ethyl palmitate, ethyl stearate, and ethyl oleate. The relationships of individual and total FAEE concentrations with BAL using albumin as a covariate were analyzed. Values for Pearson's r between the BAL and the natural log-transformed FAEE (ln FAEE) levels were calculated, and then compared with partial correlation coefficients when controlling for albumin. The impact of serum albumin levels on the relationship between ln FAEE and BAL was evaluated by simple linear regression analysis.. Concentrations of total FAEE ranged from 632 to 20,166 nM, and BAL ranged from 9 to 375 mg/dL. In the 18 samples, the Pearson correlation coefficient between BAL and total FAEE levels was 0.868, and increased to 0.909 when controlling for albumin (p<0.0001). Similar statistically significant increases in the partial correlation coefficient occurred for each of the individual species of FAEE when controlling for albumin. In simple linear regression, albumin significantly reduced the variability in the model correlating BAL and FAEE for each of the individual species, as well as for total FAEE.. The association between FAEE levels and BAL is enhanced by consideration of serum albumin concentrations. Our findings suggest that serum albumin should be included in any model attempting to define the relationship between BAL and FAEE. Topics: Alcohol Drinking; Ethanol; Humans; Linear Models; Liver; Oleic Acids; Palmitic Acids; Serum Albumin; Stearates | 2007 |
Effect of hair care and hair cosmetics on the concentrations of fatty acid ethyl esters in hair as markers of chronically elevated alcohol consumption.
Fatty acid ethyl esters (FAEE) can be used as alcohol markers in hair. It was investigated in this study whether this diagnostic method is disturbed by hair care and hair cosmetics. Traces of ethyl myristate, ethyl palmitate, ethyl oleate and ethyl stearate were detected in all of 49 frequently applied hair care products by headspace solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). The highest concentration was 0.003% in a hair wax. From experiments with separated hair samples of alcoholics as well as from the evaluation of the FAEE concentrations and the data about hair care of 75 volunteers (alcoholics, social drinkers and teetotalers) follows that usual shampooing, permanent wave, dyeing, bleaching or shading are of minor importance as compared to the drinking amount and other individual features. However, false positive results were found after daily treatment with a hair lotion containing 62.5% ethanol, with a deodorant and with a hair spray. As an explanation, it is assumed that FAEE are formed in the sebum glands also after regular topical application of products with a higher ethanol content. Topics: Alcohol Drinking; Alcoholism; Biomarkers; Deodorants; Ethyl Ethers; False Positive Reactions; Fatty Acids; Forensic Medicine; Gas Chromatography-Mass Spectrometry; Hair; Hair Preparations; Humans; Myristates; Oleic Acids; Palmitic Acids; Sebaceous Glands; Stearates; Substance Abuse Detection | 2003 |
Neonatal hair analysis as a biomarker for in utero alcohol exposure.
Topics: Alcohol Drinking; Biomarkers; Cocaine; Fatty Acids; Female; Hair; Humans; Infant, Newborn; Maternal Exposure; Myristates; Oleic Acids; Palmitic Acids; Stearates | 2002 |
Fatty acid ethyl esters in hair as markers of alcohol consumption. Segmental hair analysis of alcoholics, social drinkers, and teetotalers.
Fatty acid ethyl esters (FAEEs) are products of nonoxidative ethanol metabolism. After incorporation in hair, they should be suitable long-term markers of alcohol abuse.. Hair samples from 19 alcoholics in a treatment program, 10 fatalities with verified excessive alcohol consumption, 13 moderate social drinkers who consumed up to 20 g ethanol/day, and 5 strict teetotalers were analyzed in 1-12 segments for four FAEEs (ethyl myristate, ethyl palmitate, ethyl oleate, and ethyl stearate) by external degreasing with n-heptane, extraction with a dimethyl sulfoxide-n-heptane mixture, headspace solid-phase microextraction of the extracts, and gas chromatography-mass spectrometry with deuterated internal standards. The n-heptane washings were analyzed in the same way for FAEEs from the hair surface.. The sum of the four ester concentrations in hair calculated for the proximal 0-6 cm segment was 2.5-13.5 ng/mg (mean, 6.8 ng/mg) for the fatalities, 0.92-11.6 ng/mg (mean, 4.0 ng/mg) for 17 of the alcoholics in treatment, 0.20-0.85 ng/mg (mean, 0.41 ng/mg) for the moderate social drinkers, and 0.06-0.37 ng/mg (mean, 0.16 ng/mg) for the teetotalers. In almost all cases the segmental concentrations increased from proximal to distal. There was no agreement between the self-reported drinking histories of the participants and the FAEE concentrations along the hair length. Ethyl oleate was the dominant ester in all samples.. FAEEs are deposited in hair mainly from sebum. Despite large individual differences, FAEE hair concentrations can be used as markers for excessive alcohol consumption with relatively high accuracy. Topics: Alcohol Drinking; Alcoholic Intoxication; Alcoholism; Biomarkers; Fatty Acids; Gas Chromatography-Mass Spectrometry; Hair; Humans; Myristates; Oleic Acids; Palmitic Acids; Stearates; Substance Abuse Detection | 2001 |
Differences in the fatty acid composition of fatty acid ethyl esters in organs and their secretions.
Fatty acid ethyl esters (FAEE) are nonoxidative ethanol metabolites that have been shown to be long term markers of ethanol intake and have been implicated as mediators of ethanol-induced cell injury. Previous studies have indicated that the fatty acid composition of the FAEE found in the plasma of human subjects after ethanol ingestion is predominantly ethyl palmitate and ethyl oleate. This raised the possibility that there is some selectivity toward the fatty acid used for FAEE to be exported from the liver into the blood.. To address the hypothesis that the fatty acid composition of FAEE secreted from organs, such as the liver and pancreas, differs from the fatty acid composition of FAEE in the organs, this study was performed using rats that received ethanol by intra-arterial infusion.. It was found that the fatty acids in FAEE differed significantly in plasma versus liver, bile versus liver, and pancreatic secretions versus pancreas.. These results indicate that organs selectively export certain FAEE species. Topics: Animals; Bile; Esters; Fatty Acids; Linoleic Acids; Liver; Male; Oleic Acids; Pancreas; Pancreatic Juice; Rats; Rats, Sprague-Dawley; Rats, Wistar; Stearates | 2000 |
Ethanol-induced fatty acid ethyl ester formation in vivo and in vitro in rat lung.
Fatty acid ethyl esters (FAEE) are the end products of a non-oxidative pathway for ethanol metabolism in a variety of human, rabbit, rat and murine tissues. Our objective was to determine the significance of this pathway in the metabolism of ethanol by the rat lung. In vitro, 14C-labeled ethyl oleate formation was assayed in the lung and compared with the pancreas, liver, heart and brain. Lipids were extracted with acetone, and 14C-labeled ethyl oleate was isolated and quantified by thin layer chromatography (TLC) and scintillation spectrometry. FAEE synthetic activity in the lungs (in vitro) was found to be intermediate among the organs examined. In vivo, male rats received 10% ethanol in their drinking water with or without daily i.p. injections of 4-methylpyrazole (1 mmol/kg body wt) for 15 days. Another group of male rats received 4 g/kg body wt ethanol as a 50% (v/v) solution by gavage every 12 h for 2 days. FAEE from the three organs with the highest in vitro activity for FAEE synthesis (pancreas, liver and lung) were extracted with acetone, isolated from normal lipids by TLC and separated by gas chromatography. The lung had lower FAEE-forming activity than the pancreas or the liver in the 15-day studies. However, in the 2-day study, the lung had higher activity than the liver but lower activity than the pancreas. Ethyl oleate, ethyl stearate and ethyl palmitate were the predominant FAEE formed in the intact organism. Ethanol-induced FAEE may play a role in the development of alcohol-related injuries to the lung. Topics: Animals; Esterification; Ethanol; Fomepizole; Gas Chromatography-Mass Spectrometry; In Vitro Techniques; Linoleic Acids; Liver; Lung; Male; Oleic Acids; Palmitates; Pancreas; Pyrazoles; Rats; Rats, Inbred Strains; Stearates | 1991 |