sq-23377 and hyperforin

sq-23377 has been researched along with hyperforin* in 1 studies

Other Studies

1 other study(ies) available for sq-23377 and hyperforin

ArticleYear
Hyperforin changes the zinc-storage capacities of brain cells.
    Neuropharmacology, 2011, Volume: 61, Issue:8

    In vitro and in vivo experiments were carried out to investigate the consequences on brain cells of a chronic treatment with hyperforin, a plant extract known to dissipate the mitochondrial membrane potential and to release Zn(2+) and Ca(2+) from these organelles. Dissociated cortical neurons were grown in a culture medium supplemented with 1 μM hyperforin. Live-cell imaging experiments with the fluorescent probes FluoZin-3 and Fluo-4 show that a 3 day-hyperforin treatment diminishes the size of the hyperforin-sensitive pools of Ca(2+) and Zn(2+) whereas it increases the size of the DTDP-sensitive pool of Zn(2+) without affecting the ionomycin-sensitive pool of Ca(2+). When assayed by quantitative PCR the levels of mRNA coding for metallothioneins (MTs) I, II and III were increased in cortical neurons after a 3 day-hyperforin treatment. This was prevented by the zinc chelator TPEN, indicating that the plant extract controls the expression of MTs in a zinc-dependent manner. Brains of adult mice who received a daily injection (i.p.) of hyperforin (4 mg/kg/day) for 4 weeks had a higher sulphur content than control animals. They also exhibited an enhanced expression of the genes coding for MTs. However, the long-term treatment did not affect the brain levels of calcium and zinc. Based on these results showing that hyperforin influences the size of the internal pools of Zn(2+), the expression of MTs and the brain cellular sulphur content, it is proposed that hyperforin changes the Zn-storage capacity of brain cells and interferes with their thiol status.

    Topics: 2,2'-Dipyridyl; Animals; Brain; Calcium; Cells, Cultured; Chelating Agents; Disulfides; Dose-Response Relationship, Drug; Ethylenediamines; Gene Expression Regulation; Ionomycin; Male; Matrix Metalloproteinases; Mice; Mice, Inbred C57BL; Mitochondria; Neurons; Oxidants; Phloroglucinol; Sulfur; Terpenes; Time Factors; Zinc

2011