sq-23377 and fropenem

sq-23377 has been researched along with fropenem* in 1 studies

Other Studies

1 other study(ies) available for sq-23377 and fropenem

ArticleYear
Faropenem enhances superoxide anion production by human neutrophils in vitro.
    The Journal of antimicrobial chemotherapy, 1999, Volume: 44, Issue:3

    Neutrophils are important cellular components in the defence against infections and many studies in vitro have shown that some antibiotics affect neutrophil function. We examined the effect of faropenem, a new oral penem antibiotic on neutrophil killing function by determining the generation of superoxide anion in vitro. The production of superoxide anion was measured by chemiluminescence amplified by a Cypridina luciferin analogue in the presence of N-formyl-Met-Leu-Phe (fMLP). Faropenem significantly enhanced chemiluminescence in a dose-dependent manner. The effect of faropenem was maximal at 5 min of incubation time and continued for at least 30 min. The effect of faropenem was also observed when neutrophils were stimulated by a calcium ionophore (ionomycin), while the effect of faropenem did not change in the presence of 12-O-tetra-decanoylphorbolmyristate acetate. Cytosol Ca2+ concentration ([Ca2+]i) monitored with Fura-2 increased in response to fMLP, however, faropenem did not influence the response of [Ca2+]i to fMLP. Our results suggest that faropenem enhanced the generation of superoxide anion by neutrophils, probably at the site where cytosol Ca2+ regulates NADPH oxidase. Faropenem might be potentially advantageous in the treatment of infections because a synergic interaction of antibodies and cytocidal neutrophils is necessary for the early eradication of the pathogenic bacteria.

    Topics: Anti-Bacterial Agents; beta-Lactams; Calcium; Dose-Response Relationship, Drug; Firefly Luciferin; Humans; Ionomycin; Lactams; Luminescent Measurements; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Superoxides; Time Factors

1999