sq-23377 has been researched along with coelenterazine* in 1 studies
1 other study(ies) available for sq-23377 and coelenterazine
| Article | Year |
|---|---|
Recombinant apoaequorin acting as a pseudo-luciferase reports micromolar changes in the endoplasmic reticulum free Ca2+ of intact cells.
We describe a novel method to monitor the endoplasmic reticulum (ER) free Ca2+ in intact cells. Continuous perfusion of HeLa cells, expressing ER-targeted apoaequorin, with coelenterazine allowed the apoprotein to act as a pseudo-luciferase capable of reporting free Ca2+ from 0.1-100 microM. In intact HeLa cells, addition of ionomycin increased apoaequorin-generated light by 91%, indicating that resting ER free Ca2+ was approx. 2 microM. Agonist stimulation decreased the ER apoaequorin signal and proportionally increased cytosolic free Ca2+ consistent with agonist-induced release of Ca2+ from the ER. Topics: Adenosine Triphosphate; Aequorin; Apoproteins; Calcium; Endoplasmic Reticulum; HeLa Cells; Humans; Imidazoles; Ionomycin; Kinetics; Luciferases; Luminescent Measurements; Pyrazines; Recombinant Proteins; Sensitivity and Specificity; Time Factors | 1996 |