sq-23377 and calcium-orange

sq-23377 has been researched along with calcium-orange* in 1 studies

Other Studies

1 other study(ies) available for sq-23377 and calcium-orange

ArticleYear
Monitoring cytosolic calcium in the dinoflagellate Crypthecodinium cohnii with calcium orange-AM.
    Plant & cell physiology, 2005, Volume: 46, Issue:6

    Calcium plays several important roles in the signal transduction pathways of dinoflagellates. We describe here the development of calcium orange-AM as an intracellular calcium reporter for the heterotrophic dinoflagellate Crypthecodinium cohnii. We demonstrated with confocal microscopy that by restricting the incubation period to 30-45 min, no compartmentalization of the dye occurs in the mitochondria or endoplasmic reticulum. The dye fluorescence responded well to the effects of calcium ionophores and calcium chelators. By calibrating the dye with known calcium concentrations, we determined the intracellular calcium concentration of C. cohnii to be 158 +/- 56 nM, which rose to about 550 nM upon mechanical stimulation.

    Topics: Animals; Calcimycin; Calcium; Chelating Agents; Cytosol; Dinoflagellida; Egtazic Acid; Fluorescent Dyes; Ionomycin; Ionophores; Microscopy, Confocal; Organic Chemicals; Stress, Mechanical

2005