sq-23377 and 8-thioguanosine

CD2 is expressed on murine B cells, probably as a result of a chromosomal translocation event during speciation. There are no activating antibodies to mouse CD2, but when activated non-specifically, only T cells up-regulate their expression of CD2. We investigated the expression and function of CD2 on B cells using mice transgenic for human CD2 under the control of a modified version of the autologous promoter/enhancer that included the immunoglobulin enhancer. This construction directs expression of human CD2 to the B cell compartment as well as to the T cell compartment. In this paper, we confirm that activating pairs of anti-CD2 monoclonal antibodies are mitogenic for mouse T cells transgenic for human CD2. In contrast, mouse B cells that express similar amounts of human CD2 are not stimulated to proliferate by equivalent doses of these antibodies. We were also unable to show any functional consequence for these B cells as a result of CD2 ligation.

Topics: Adjuvants, Immunologic; Animals; Antibodies, Monoclonal; B-Lymphocytes; CD2 Antigens; CD40 Antigens; Cell Division; Cells, Cultured; Gene Expression Regulation; Genes, MHC Class II; Guanosine; Humans; Immunoglobulin Heavy Chains; Immunoglobulin M; Ionomycin; Lipopolysaccharides; Mice; Mice, Transgenic; Promoter Regions, Genetic; T-Lymphocytes; Thionucleosides