sq-23377 has been researched along with 2--7--dichlorofluorescein* in 2 studies
2 other study(ies) available for sq-23377 and 2--7--dichlorofluorescein
| Article | Year |
|---|---|
Attenuation of Ca(2+)-induced increase in oxidative metabolism by cooling and calmodulin antagonist in mammalian brain neurons: a flow-cytometric study.
Effects of cooling and calmodulin antagonist on ionomycin-induced increase in oxidative metabolism (or formation of reactive oxygen species) of rat cerebellar neurons was examined using a flow cytometer and 2',7'-dichlorofluorescin diacetate, a fluorescent dye for intracellular hydrogen peroxide. Cooling neurons to temperatures below 16 degrees C greatly attenuated ionomycin-induced augmentation of oxidative metabolism without affecting the Ca2+ influx produced by ionomycin. Rewarming neurons to 36 degrees C in presence of ionomycin increased the oxidative metabolism, indicating a temperature-sensitive metabolic process. Substitution of Ca2+ with Ba2+ or Sr2+ completely abolished an ionomycin-induced increase in the oxidative metabolism. Pretreatment with W-7, a calmodulin antagonist, at concentrations of 10 microM or higher (up to 100 microM) produced a dose-dependent attenuation of ionomycin-induced increase in oxidative metabolism. Results suggest that calmodulin is involved in the ionomycin-induced increase in oxidative metabolism of dissociated cerebellar neurons. Topics: Animals; Brain; Calcium; Calmodulin; Cold Temperature; Flow Cytometry; Fluoresceins; Ionomycin; Neurons; Oxidation-Reduction; Rats; Rats, Wistar; Sulfonamides | 1994 |
Ca(2+)-induced increase in oxidative metabolism of dissociated mammalian brain neurons: effect of extract of ginkgo biloba leaves.
Effect of an extract of Ginkgo biloba leaves (EGb) on oxidative metabolism was studied using rat brain neurons and 2',7'-dichlorofluorescin fluorescence. Ionomycin (100 nM to 1 microM), a Ca(2+)-ionophore, dose-dependently augmented the 2',7'-dichlorofluorescin fluorescence in the presence of external Ca2+, but not under the external Ca(2+)-free condition. Preincubation of neurons with EGb (3 micrograms/ml) greatly reduced the ionomycin-induced increase in 2',7'-dichlorofluorescin fluorescence. Results suggest that EGb may reduce the Ca(2+)-induced increase in the oxidative metabolism of brain neurons. Topics: Animals; Brain Chemistry; Calcium; Cerebellum; Fluoresceins; In Vitro Techniques; Ionomycin; Neurons; Oxidation-Reduction; Plant Extracts; Rats; Rats, Inbred WKY | 1993 |