sphingosine-kinase and icaritin

sphingosine-kinase has been researched along with icaritin* in 1 studies

Other Studies

1 other study(ies) available for sphingosine-kinase and icaritin

ArticleYear
Identification of sphingosine kinase 1 (SphK1) as a primary target of icaritin in hepatocellular carcinoma cells.
    Oncotarget, 2017, Apr-04, Volume: 8, Issue:14

    Hepatocellular carcinoma (HCC) is a highly aggressive neoplasm. We aim to explore the anti-HCC activity by a natural prenylflavonoid icaritin. Icaritin was cytotoxic and pro-apoptotic when added to established (HepG2, KYN-2 and Huh-7 lines) and primary human HCC cells. At the signaling level, icaritin inhibited sphingosine kinase 1 (SphK1) activity in HCC cells, which led to pro-apoptotic ceramide production and JNK1 activation. SphK1 inhibition or silence (by shRNA/microRNA) mimicked icaritin-mediated cytotoxicity, and almost nullified icaritin's activity in HepG2 cells. Reversely, exogenous over-expression of SphK1 sensitized icaritin-induced HepG2 cell apoptosis. In vivo, oral administration of icaritin dramatically inhibited HepG2 xenograft growth in SCID mice. Further, SphK1 activity in icaritin-treated tumors was largely inhibited. In summary, icaritin exerts potent anti-HCC activity in vitro and in vivo. SphK1 inhibition could be the primary mechanism of its actions in HCC cells.

    Topics: Animals; Apoptosis; Biomarkers, Tumor; Carcinoma, Hepatocellular; Cell Proliferation; Ceramides; Flavonoids; Gene Expression Regulation, Enzymologic; Humans; Liver Neoplasms; Male; Mice; Mice, SCID; Middle Aged; Phosphotransferases (Alcohol Group Acceptor); Signal Transduction; Tumor Cells, Cultured; Xenograft Model Antitumor Assays

2017