sphingosine-kinase and 4-nitrobenzofurazan

sphingosine-kinase has been researched along with 4-nitrobenzofurazan* in 1 studies

Other Studies

1 other study(ies) available for sphingosine-kinase and 4-nitrobenzofurazan

Article	Year
NBD-labeled derivatives of the immunomodulatory drug FTY720 as tools for metabolism and mode of action studies. Bioorganic & medicinal chemistry letters, 2006, Jan-01, Volume: 16, Issue:1 Fluorescently labeled chiral analogs of the immunomodulatory drug FTY720 and its corresponding phosphates with variable aliphatic spacers between the aromatic ring and the NBD label have been synthesized. Determining the influence of the spacer on the in vitro phosphorylation rate by SPHK1 and 2 resulted in the identification of NBD-(R)-AAL 1c,d which are phosphorylated with an efficiency comparable to that of the unlabeled FTY720 analog (R)-AAL. Furthermore, the NBD-(R)-AAL phosphates 10c,d were proven to be a functional S1P receptor agonist. Topics: Calcium; Cell Line; Cells, Cultured; Dose-Response Relationship, Drug; Endothelium, Vascular; Fingolimod Hydrochloride; Flow Cytometry; Humans; Immunosuppressive Agents; Lysophospholipids; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Models, Chemical; Oxadiazoles; Phosphorylation; Phosphotransferases (Alcohol Group Acceptor); Propylene Glycols; Protein Binding; Receptors, Lysosphingolipid; Recombinant Proteins; Sphingosine; Substrate Specificity; Time Factors	2006

Article

Year

NBD-labeled derivatives of the immunomodulatory drug FTY720 as tools for metabolism and mode of action studies.

Bioorganic & medicinal chemistry letters, 2006, Jan-01, Volume: 16, Issue:1

Fluorescently labeled chiral analogs of the immunomodulatory drug FTY720 and its corresponding phosphates with variable aliphatic spacers between the aromatic ring and the NBD label have been synthesized. Determining the influence of the spacer on the in vitro phosphorylation rate by SPHK1 and 2 resulted in the identification of NBD-(R)-AAL 1c,d which are phosphorylated with an efficiency comparable to that of the unlabeled FTY720 analog (R)-AAL. Furthermore, the NBD-(R)-AAL phosphates 10c,d were proven to be a functional S1P receptor agonist.

Topics: Calcium; Cell Line; Cells, Cultured; Dose-Response Relationship, Drug; Endothelium, Vascular; Fingolimod Hydrochloride; Flow Cytometry; Humans; Immunosuppressive Agents; Lysophospholipids; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Models, Chemical; Oxadiazoles; Phosphorylation; Phosphotransferases (Alcohol Group Acceptor); Propylene Glycols; Protein Binding; Receptors, Lysosphingolipid; Recombinant Proteins; Sphingosine; Substrate Specificity; Time Factors

2006