sorivudine and 5-propynylarabinofuranosyluracil

sorivudine has been researched along with 5-propynylarabinofuranosyluracil* in 3 studies

Reviews

1 review(s) available for sorivudine and 5-propynylarabinofuranosyluracil

ArticleYear
Successors to acyclovir.
    The Journal of antimicrobial chemotherapy, 1994, Volume: 34, Issue:3

    Topics: 2-Aminopurine; Acyclovir; Antiviral Agents; Arabinofuranosyluracil; Chickenpox; Drug Resistance, Microbial; Famciclovir; Herpes Simplex; Herpes Zoster; Humans; Valacyclovir; Valine

1994

Other Studies

2 other study(ies) available for sorivudine and 5-propynylarabinofuranosyluracil

ArticleYear
Construction of a herpes simplex virus/varicella-zoster virus (HSV/VZV) thymidine kinase recombinant with the pathogenic potential of HSV and a drug sensitivity profile resembling that of VZV.
    The Journal of general virology, 1995, Volume: 76 ( Pt 8)

    A recombinant of herpes simplex virus (HSV) was constructed in which the HSV thymidine kinase (TK) gene was deleted and the varicella-zoster virus (VZV) TK gene was introduced into the US5 region under the control of the human cytomegalovirus IE promoter. Infection with the recombinant (R18) led to the induction of TK although the kinetics of synthesis resembled those of a 'late' gene product. The recombinant was virulent in the zosteriform mouse model with the pattern of pathogenesis similar to that of wild-type HSV-1. The sensitivity of the recombinant to several nucleoside analogues was assessed and in most cases (BVaraU, ACV and GCV) it resembled VZV rather than HSV. The enhanced sensitivity of the recombinant to BVaraU compared with wild-type HSV resulted in a far greater response to treatment with BVaraU as assessed in the mouse model.

    Topics: Acyclovir; Animals; Antiviral Agents; Arabinofuranosyluracil; Base Sequence; Cytomegalovirus; Drug Evaluation, Preclinical; Ganciclovir; Herpes Simplex; Herpesvirus 1, Human; Herpesvirus 3, Human; Humans; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Promoter Regions, Genetic; Thymidine Kinase; Transformation, Genetic; Virulence; Virus Latency

1995
Analysis of mutations in the thymidine kinase genes of drug-resistant varicella-zoster virus populations using the polymerase chain reaction.
    The Journal of general virology, 1991, Volume: 72 ( Pt 3)

    We have applied the polymerase chain reaction (PCR) technique to analyse mutations in the thymidine kinase (TK) gene of varicella-zoster virus (VZV) associated with resistance to the 5-bromovinyl (BVaraU) and 5-propynyl (PYaraU) analogues of arabinofuranosyl deoxyuridine. The results from this study allow three clear conclusions to be drawn. Firstly, the technique clearly shows that populations of VZV derived from plaque purification were truly clonal only when the plaques were initiated from cell-free virus (representing a tiny fraction of infectious virus) and plaques initiated by infected cells contained a mixture of variants. Secondly, despite the background mutations caused by errors of the Taq DNA polymerase, mutations relevant to drug resistance can easily be distinguished. The BVaraU-resistant mutant, 7-1, contained an aspartic acid to asparagine mutation at residue 18 and a single base deletion (position 65298 of the VZV DNA sequence), resulting in a frameshift and premature termination of the polypeptide chain, was found in the BVaraU-resistant mutant YSR. PYaraU-resistant virus populations contained viruses with one or more of three independent mutations, i.e. single base substitutions resulting in mutations from leucine to proline at residue 92, histidine to arginine at residue 97 and a deletion of 20bp (residues 65,135 to 65,154). Finally, the technique has uncovered novel sites in the virus TK associated with drug resistance. We conclude that in vitro amplification using the PCR combined with cloning and sequencing is a relatively rapid method for identifying mutations in small virus populations even when they are not homogeneous.

    Topics: Amino Acid Sequence; Animals; Antiviral Agents; Arabinofuranosyluracil; Base Sequence; Cell Line; Cloning, Molecular; DNA, Viral; Drug Resistance, Microbial; Electrophoresis, Polyacrylamide Gel; Herpesvirus 3, Human; Humans; Molecular Sequence Data; Mutation; Polymerase Chain Reaction; Thymidine Kinase; Vero Cells

1991