sophoricoside has been researched along with orobol* in 2 studies
2 other study(ies) available for sophoricoside and orobol
Article | Year |
---|---|
Differential inhibitory effects of sophoricoside analogs on bioactivity of several cytokines.
Effects of sophoricoside and its analogs on proinflammatory cytokines have been investigated. Sophoricoside, genistein and orobol exhibited inhibitory effects on IL-5, IL-3, GM-CSF and IL-6 bioactivities. Genistin showed inhibitory effects on IL-5 and IL-3 bioactivities, but did not inhibit GM-CSF and IL-6 bioactivities. None of the sophoricoside analogs showed inhibitory effects on both IL-1beta and TNF-alpha bioactivities. Among the compounds, sophoricoside exhibited the highest inhibitory effects on IL-5, IL-3 and IL-6 bioactivities with IC50 values of 1.9 microM, 6.9 microM and 6.0 microM, respectively and orobol did show on GM-CSF bioactivity with an IC50 value of 18.0 microM. The result would provide an additional mechanism by which the compounds exert immunosuppressive and anti-inflammatory effects. Topics: Animals; B-Lymphocytes; Benzopyrans; Cell Division; Cell Line; Cytokines; Flavonoids; Genistein; Granulocyte-Macrophage Colony-Stimulating Factor; Humans; Interleukin-1; Interleukin-3; Interleukin-5; Interleukin-6; Isoflavones; Leukemia, Erythroblastic, Acute; Melanoma; Mice; Recombinant Proteins; Structure-Activity Relationship; Tumor Cells, Cultured; Tumor Necrosis Factor-alpha | 2000 |
Sophoricoside analogs as the IL-5 inhibitors from Sophora japonica.
Fruits of Sophora japonica L. (Leguminosae) exhibited an inhibitory effect in the IL-5 bioassay of mIL-5-dependent Y16 proliferation. The isoflavonoids of sophoricoside, genistein, orobol, and genistin were isolated as the IL-5 inhibitors from fresh fruits of the plant by activity-guided fractionation. Among the IL-5 inhibitors, sophoricoside exhibited the highest inhibitory effect with 89% inhibition at 12.5 microM, 82% at 6.3 microM, 72% at 3.1 microM, 59% at 1.6 microM, and 24% at 0.8 microM where the 50% inhibition (IC50) was shown at the concentration of 1.5 microM. Oxyphenylbutazone as the positive control exhibited the IC50 value at the concentration of 31.7 microM. In the order of IC50 values the inhibitory potency in the IL-5 bioassay was sophoricoside > orobol (9.8 microM) approximately equal to genistin (10.6 microM) > genistein (51.9 microM). The position of O-glycosylation and number of hydroxy groups in the isoflavonoids seem to play an important role in the inhibitory effect in the IL-5 bioassay. Topics: Animals; B-Lymphocytes; Benzopyrans; Cell Line; Enzyme Inhibitors; Fabaceae; Flavonoids; Genistein; Interleukin-5; Mice; Molecular Structure; Plant Extracts; Plants, Medicinal | 1999 |