songorine and napelline

A new denudatine-type C

Topics: Aconitine; Aconitum; Alkaloids; Diterpenes; Molecular Structure; Plant Extracts; Spectrum Analysis

We compared analgesic activities of individual alkaloids extracted from Baikal aconite (Aconitum baikalensis): napelline, hypaconitine, songorine, mesaconitine, 12-epinapelline N-oxide. The detected analgesic activity was comparable to that of sodium metamizole. The mechanisms of analgesia were different in diterpene alkaloids of different structure. The antinociceptive effect of atisine alkaloids (12-epinapelline N-oxide, songorine) was naloxonedependent and realized via opioid receptor modulation.

Topics: Acetic Acid; Aconitine; Aconitum; Alkaloids; Analgesics; Animals; Animals, Outbred Strains; Arthritis, Experimental; Dipyrone; Freund's Adjuvant; Injections, Intraperitoneal; Mice; Pain; Plant Extracts; Rats; Seizures; Vocalization, Animal

The effects of complex extract from Aconitum baikalense on reparative regeneration of a plane dorsal skin wound were studied. Treatment with Aconitum baikalense tincture stimulated reparation and skin regeneration. The effects of the Aconitum baikalense alkaloids on functional activity of fibroblast precursors were studied in vitro by cultural methods. Mesaconitine, hypaconitine, songorine, napelline, and 12-epinapelline N-oxide significantly stimulated the growth of colonies from fibroblast precursors. This indicated direct stimulation of fibroblasts by aconite alkaloids, which could be a mechanism of reparative activity of the complex extract.

Topics: Aconitine; Aconitum; Alkaloids; Animals; Cell Proliferation; Cells, Cultured; Colony-Forming Units Assay; Female; Fibroblasts; Mice; Mice, Inbred CBA; Plant Components, Aerial; Plant Extracts; Regeneration; Skin; Wound Healing

Regeneratory activities of Baikal aconite alkaloids were studied on the excision skin wound model. Manifest wound healing effects of songorine, napelline, and hypaconitine were detected. The therapeutic efficiency was based on activation of residual mesenchymal progenitor elements. The development of this phenomenon was explained by the direct effects of alkaloids on precursors and by higher production of growth factors by the skin stromal cells. Songorine exhibited the most pronounced specific activity due to more significant stimulation of progenitor cell differentiation associated with maximum activation of the secretory function of the microenvironment cells.

Topics: Aconitine; Aconitum; Alkaloids; Animals; Animals, Outbred Strains; Cell Differentiation; Cellular Microenvironment; Diterpenes; Intercellular Signaling Peptides and Proteins; Male; Mesenchymal Stem Cells; Mice; Regeneration; Skin; Stromal Cells; Wound Healing; Wounds and Injuries