sodium-perchlorate and guanosine-5--phospho-2-methylimidazolide

Because of the stable self-structures formed by oligomers of guanosine, standard high-performance liquid chromatography techniques for oligonucleotide fractionation are not applicable. Previously, oligoguanylate separations have been carried out at pH 12 using RPC-5 as the packing material. While RPC-5 provides excellent separations, there are several limitations, including the lack of a commercially available source. This report describes a new anion-exchange high-performance liquid chromatography method using HEMA-IEC BIO Q, which successfully separates different forms of the guanosine monomer as well as longer oligoguanylates. The reproducibility and stability at high pH suggests a versatile role for this material.

Topics: Chromatography, High Pressure Liquid; Guanosine; Guanosine Monophosphate; Hydrogen-Ion Concentration; Oligonucleotides; Perchlorates; Poly C; Poly G; Silicon Dioxide; Sodium Compounds