sodium-perchlorate and cumene-hydroperoxide

The carbon centered radicals generated by preincubation of cumene hydroperoxide with rat cardiac membranes dose dependently reduced the Bmax of [3H]nitrendipine binding, while KD was unchanged. The reduction induced by cumene hydroperoxide was prevented by 2,6-di-t-butlyl-4-methyl-phenol. The generation of OH did not influence the [3H]nitrendipine binding; also other oxidative agents (H2O2 and HClO4) did not modify this binding. Therefore the reduction in [3H]nitrendipine binding sites is not attributable to generic oxidative stress but to the formation of carbon centered radicals.

Topics: Animals; Benzene Derivatives; Butylated Hydroxytoluene; Calcium Channels; Calcium Channels, L-Type; Cell Membrane; Down-Regulation; Electron Spin Resonance Spectroscopy; Free Radicals; Hydrogen Peroxide; Kinetics; Male; Myocardium; Nitrendipine; Oxidants; Oxidative Stress; Perchlorates; Rats; Rats, Sprague-Dawley; Sodium Compounds; Superoxides