sodium-nitrite and sodium-sulfide

Saccharomyces cerevisiae showed an ultradian respiratory oscillation during aerobic continuous culture. Analysis of the off-gas revealed that hydrogen sulphide production also oscillated. Production was first detected at the onset of low respiration and reached a maximum (1.5 microM) prior to minimum respiratory activity. Then H(2)S concentration fell rapidly to below 0.2 microM before the onset of high respiration. Injection of respiratory oscillation perturbation agents, such as glutathione (50 microM), NaNO(2) (50 microM) or acetaldehyde (4.5 mM),() transiently increased H(2)S production above 6 microM. The synchronization properties of H(2)S were analysed to reveal that changes of oscillation period and amplitude were dependent on H(2)S concentration in culture. It is concluded that H(2)S produced during oscillation produces population synchrony by respiratory chain inhibition.

Topics: Acetaldehyde; Biological Clocks; Dose-Response Relationship, Drug; Glutathione; Hydrogen Sulfide; Oxygen Consumption; Saccharomyces cerevisiae; Sodium Nitrite; Sulfides

In order to evaluate the efficiency of hyperbaric oxygen in experimental acute sulfide poisoning, we studied the effect of 1 ATA (atmosphere absolute) oxygen and sodium nitrite therapy. We then studied the effect of oxygen at 3 ATA alone and in combination with intraperitoneal sodium nitrite injection on rats poisoned by intraperitoneal injection of LD75 sulfide. Electroencephalogram and heart rate were continuously monitored. We also studied the effect of sodium nitrite and hyperbaric oxygen administered before the poisoning (protective effect). In our experimental set, death of untreated poisoned animals occurred within 5 min. There is a parallel between modification of the EEG pattern and apnea. Respiratory arrest always preceded cardiac arrest. Pure oxygen (1 ATA O2) is effective in preventing death in experimental sulfide poisoning. 3 ATA oxygen was significantly more effective in preventing death than 1 ATA oxygen, or sodium nitrite alone. The best therapeutic regimen was a combination of 3 ATA oxygen and sodium nitrite administration.

Topics: Animals; Apnea; Electrocardiography; Electroencephalography; Heart Rate; Hyperbaric Oxygenation; Injections, Intraperitoneal; Lethal Dose 50; Male; Rats; Respiratory Insufficiency; Sodium Nitrite; Sulfides