sodium-nitrite and isoascorbic-acid

Topics: Antioxidants; Ascorbic Acid; Food Microbiology; Food Preservatives; Hydroxylamines; Lactobacillus delbrueckii; Meat Products; Mutagens; Nitriles; Pyrroles; Sodium Nitrite; Sorbic Acid

The effect of nitrite and erythorbate on Clostridium perfringens spore germination and outgrowth in ham during abusive cooling (15 h) was evaluated. Ham was formulated with ground pork, NaNO2 (0, 50, 100, 150 or 200 ppm) and sodium erythorbate (0 or 547 ppm). Ten grams of meat (stored at 5 °C for 3 or 24 h after preparation) were transferred to a vacuum bag and inoculated with a three-strain C. perfringens spore cocktail to obtain an inoculum of ca. 2.5 log spores/g. The bags were vacuum-sealed, and the meat was heat treated (75 °C, 20 min) and cooled within 15 h from 54.4 to 7.2 °C. Residual nitrite was determined before and after heat treatment using ion chromatography with colorimetric detection. Cooling of ham (control) stored for 3 and 24 h, resulted in C. perfringens population increases of 1.46 and 4.20 log CFU/g, respectively. For samples that contained low NaNO2 concentrations and were stored for 3 h, C. perfringens populations of 5.22 and 2.83 log CFU/g were observed with or without sodium erythorbate, respectively. Residual nitrite was stable (p > 0.05) for both storage times. Meat processing ingredients (sodium nitrite and sodium erythorbate) and their concentrations, and storage time subsequent to preparation of meat (oxygen content) affect C. perfringens spore germination and outgrowth during abusive cooling of ham.

Topics: Animals; Ascorbic Acid; Clostridium perfringens; Colony Count, Microbial; Food Preservation; Food Preservatives; Meat Products; Sodium Nitrite; Spores, Bacterial; Swine; Temperature; Vacuum

The growth of Bacillus cereus is a problem in liver sausage especially when the sausages are stored at high temperatures. Even concentrations of greater than 10(6)/g have been detected. In this study we found that when combining glucono-delta-lactone, sodium erythorbate and citric acid with sodium nitrite and salt the growth of B. cereus could be delayed or totally inhibited.

Topics: Animals; Ascorbic Acid; Bacillus cereus; Citrates; Citric Acid; Food Microbiology; Food Preservation; Gluconates; Lactones; Liver; Meat; Meat Products; Sodium Nitrite; Swine; Temperature