sodium-nitrite and isoamylamine

Topics: Amines; Fusarium; Nitrites; Nitrosamines; Sodium Nitrite

Nitrosomethylisoamylamine (NMIA), a carcinogenic N-nitroso compound was synthesized from isoamylamine (IAA) in a glucose-ammonium nitrate medium after several days' incubation with fungi and subsequent nitrosation with sodium nitrate. The nitrosamine was not produced by control reactions which lacked either IAA or fungi. The yield of NMIA varied with the length of incubation after inoculation with the fungi, and with the concentrations of IAA and NaNO2, the duration of nitrosation, the pH of the medium and the species of the fungi. The optimum conditions for the formation of the nitrosamine are reported.

Topics: Amines; Carcinogens; Fungi; Fusarium; Hydrogen-Ion Concentration; Nitrites; Nitrosamines; Penicillium; Sodium Nitrite

Methylisoamylnitrosamine, a carcinogenic N-nitroso compound, has been formed in glucose ammonium nitrate medium containing 150 mg of isoamylamine (a primary amine) inoculated with a common fungus (Fusarium moniliforme Sheldon), to which 400 mg NaNO2 are added after incubation for 7-8 days at 28 degrees C. No such compound has been found in control samples without fungi or isoamylamine. This paper reports a method of experimental synthesis of the nitrosamine by F. moniliforme strains 82-01 and 83-01, using isoamylamine and NaNO2, as well as the analysis of the compound by GC/MS and GC/TEA.

Topics: Amines; Carcinogens; Fusarium; Nitrosamines; Sodium Nitrite