sodium-nitrite has been researched along with 7-methylguanine* in 2 studies
2 other study(ies) available for sodium-nitrite and 7-methylguanine
| Article | Year |
|---|---|
[DNA alkylation during endogenous synthesis of N-nitrosodimethylamine].
The simultaneous administration of 14C-dimethylamine and sodium nitrite to rat stomach produces the formation of O6-methylguanine and 7-methylguanine in the liver DNA as a result of the endogenic synthesis of N-nitrosodimethylamine (NDMA). Methylation of DNA was also observed in the rat kidney and lung. Alkylation of O6-position of guanine and removal of its methylated product from the DNA occurred in the liver to a greater extent than in the other NDMA target organs. Pretreatment of rats with sodium nitrite increased the alkylation of liver DNA caused by endogenic synthesis of NDMA. The formation of alkylated products in DNA may be used as a marker for the endogenic formation of NDMA from precursors. Topics: Alkylation; Animals; Dimethylamines; Dimethylnitrosamine; DNA; DNA Damage; Guanine; Kidney; Liver; Lung; Male; Rats; Sodium Nitrite | 1987 |
Measurement of 7-methylguanine as an estimate of the amount of dimethylnitrosamine formed following administration of aminopyrine and nitrite to rats.
We have demonstrated that there is a dose-related increase in the excretion of 7-[methyl-14C]methylguanine ( [14C]m7Gua) following p.o. administration of di[methyl-14C]methylnitrosamine to rats. Urine was collected for 24 hr after di[methyl-14C]methylnitrosamine administration, and the purines were precipitated from an aliquot of the urine with silver nitrate. Purines were released from the precipitate with HCl, and [14C]m7Gua was quantified by chromatography on an Aminex A-6 column. The excretion of [14C]m7Gua increased linearly with the dose of dimethylnitrosamine. This relationship was used to estimate the amount of di[methyl-14C]methylnitrosamine formed in the reaction of [14C]aminopyrine with sodium nitrite in rats gavaged with these compounds. The dose of dimethylnitrosamine was also estimated from the amount of alkylation of liver DNA in the same animals. These estimates usually differed by less than a factor of 2. [14C]aminopyrine and sodium nitrite were administered. The possibility of using this assay to obtain data on nitrosation in humans is discussed. Topics: Aminopyrine; Animals; Carbon Radioisotopes; Dimethylamines; Dimethylnitrosamine; DNA; Guanine; Kinetics; Liver; Male; Nitrites; Rats; Rats, Inbred Strains; Sodium Nitrite | 1983 |