sodium-hypochlorite and guanidine-thiocyanate

Decontamination of instruments is a prerequisite for their potential reuse but may affect surface integrity. Hence, the effect of prion removal protocols on 7 brands of nickel-titanium files was investigated. Baseline debris scores were determined under magnification after staining with van Gieson's solution. After shaping root canals in vitro, rotaries were mechanically and ultrasonically cleaned followed by immersion for 24 hours in 2 M sodium hydroxide (NaOH), 6 M CH(5)N(3), or 3% sodium hypochlorite (NaOCl); control files were stored dry. After sterilization, files were again stained and evaluated. Two of seven file brands demonstrated significantly higher baseline debris scores compared to final scores. Uniformly, debris could not be completely removed; there were no significant differences among groups. After immersion in NaOCl, 27.8% of instruments showed corrosion; however, no deterioration after immersion in the other solutions was found in the other groups. Regarding corrosion, no significant difference was found between brands. Based on these findings, single use of nickel-titanium rotaries appears beneficial.

Topics: Coloring Agents; Corrosion; Decontamination; Dental Alloys; Disinfectants; Equipment Contamination; Equipment Design; Guanidines; Humans; Indicators and Reagents; Nickel; Picrates; Prions; Root Canal Preparation; Rosaniline Dyes; Sodium Hydroxide; Sodium Hypochlorite; Sterilization; Surface Properties; Thiocyanates; Titanium; Ultrasonics

Effective reprocessing of surgical instruments ensuring elimination of inadvertent contamination with infectious agents causing transmissible spongiform encephalopathies (TSEs) is essential for the prevention of iatrogenic transmission of Creutzfeldt-Jakob disease (CJD) or its new variant (vCJD) from asymptomatic carriers. In a search for effective yet instrument-friendly and routinely applicable reprocessing procedures, we used an in vitro carrier assay to assess the decontamination activity exerted by different reagents on pathological prion protein (PrP(Sc)), the biochemical marker for TSE infectivity, attached to steel surfaces. In this assay, steel wires were contaminated with 263K scrapie brain homogenate and reprocessed for decontamination by exposure to several different test reagents. Residual contamination with PrP(Sc) and its protease-resistant core PrP27-30, still present after reprocessing on the wire surface or in the cleaning solution, was monitored by sensitive Western blot detection without or after proteinase K digestion. Using this approach, various reagents and processing conditions were screened for both their efficacy of decontamination and their active principles, such as detachment, destabilization or degradation of surface-bound prion protein. This revealed that, under appropriate conditions, relatively mild reagents such as 0.2 % SDS/0.3 % NaOH (pH 12.8), a commercially available alkaline cleaner (pH 11.9-12.2), a disinfectant containing 0.2 % peracetic acid and low concentrations of NaOH (pH 8.9) or 5 % SDS (pH 7.1) exert potent decontaminating activities on PrP(Sc)/PrP27-30 attached to steel surfaces. For in vivo validation, wires reprocessed in these reagents have been implanted into reporter animals in ongoing experiments.

Topics: Decontamination; Guanidines; PrPSc Proteins; Sodium Hydroxide; Sodium Hypochlorite; Steel; Surgical Instruments; Thiocyanates; Urea