sodium-hypochlorite and 2-2--azobis(2-amidinopropane)

sodium-hypochlorite has been researched along with 2-2--azobis(2-amidinopropane)* in 2 studies

Other Studies

2 other study(ies) available for sodium-hypochlorite and 2-2--azobis(2-amidinopropane)

Article	Year
Oxidation-induced aggregation of LDL increases their uptake by smooth muscle cells from human aorta. Bulletin of experimental biology and medicine, 2007, Volume: 143, Issue:2 Oxidative modification of human blood LDL induced by Cu2+, NaOCl, or 2,2-azobis-(2-aminopropane hydrochloride) was followed by their partial aggregation. Separation of oxidized LDL into aggregates and nonaggregated particles showed that they are characterized by a similar degree of oxidative modification. In contrast to nonaggregated particles, LDL aggregates in the same concentration significantly increased cholesterol content in smooth muscle cells from the intact (no involoved in atherosclerosis) human aortic intima. Our results indicate that atherogenicity of LDL oxidized by various factors is mainly associated with the formation of aggregates, but does not depend on the degree of oxidative modification. Topics: Amidines; Aorta; Cells, Cultured; Cholesterol; Copper Sulfate; Humans; Kinetics; Lipid Peroxidation; Lipoproteins, LDL; Myocytes, Smooth Muscle; Oxidation-Reduction; Sodium Hypochlorite	2007
Kinetics and uptake in vivo of oxidatively modified lymph chylomicrons. The American journal of physiology, 1995, Volume: 268, Issue:4 Pt 1 The metabolism of oxidized chylomicrons (ox-CMs) was investigated in vivo. CMs from rats fed corn, linseed, or fish oil were oxidized by incubation with 2,2'-azobis(2-amidinopropane)hydrochloride (AAPH) or sodium hypochlorite (NaOCl). Oxidized CMs had a rapid phase of clearance, followed by a slow phase. Clearance of ox-CMs was decreased for corn oil but increased for linseed and fish oil particles. Differences in rats of uptake between CM types or treatment were independent of the rate of remnant formation, but were instead a consequence of decreased clearance. A greater triglyceride-to-cholesteryl ester ratio in liver suggested that there was less lipolysis of ox-CM triglyceride prior to uptake. Hepatic uptake of ox-CMs was decreased, whereas there was increased uptake in spleen. However, the uptake by Kupffer cells of ox-CMs was 43% of total liver uptake after AAPH treatment and 59% after NaOCl treatment, compared with 21% for control CMs. Collectively, our data show that oxidation can have differential effects on the rate of clearance of CMs and that ox-CMs are preferentially cleared by the reticuloendothelial system. Topics: Amidines; Animals; Cholesterol Esters; Chylomicrons; Corn Oil; Dietary Fats, Unsaturated; Fish Oils; Kinetics; Kupffer Cells; Linseed Oil; Liver; Lymph; Oxidation-Reduction; Rats; Rats, Wistar; Sodium Hypochlorite; Spleen; Triglycerides	1995

Article

Year

Oxidation-induced aggregation of LDL increases their uptake by smooth muscle cells from human aorta.

Bulletin of experimental biology and medicine, 2007, Volume: 143, Issue:2

Oxidative modification of human blood LDL induced by Cu2+, NaOCl, or 2,2-azobis-(2-aminopropane hydrochloride) was followed by their partial aggregation. Separation of oxidized LDL into aggregates and nonaggregated particles showed that they are characterized by a similar degree of oxidative modification. In contrast to nonaggregated particles, LDL aggregates in the same concentration significantly increased cholesterol content in smooth muscle cells from the intact (no involoved in atherosclerosis) human aortic intima. Our results indicate that atherogenicity of LDL oxidized by various factors is mainly associated with the formation of aggregates, but does not depend on the degree of oxidative modification.

Topics: Amidines; Aorta; Cells, Cultured; Cholesterol; Copper Sulfate; Humans; Kinetics; Lipid Peroxidation; Lipoproteins, LDL; Myocytes, Smooth Muscle; Oxidation-Reduction; Sodium Hypochlorite

2007

Kinetics and uptake in vivo of oxidatively modified lymph chylomicrons.

The American journal of physiology, 1995, Volume: 268, Issue:4 Pt 1

The metabolism of oxidized chylomicrons (ox-CMs) was investigated in vivo. CMs from rats fed corn, linseed, or fish oil were oxidized by incubation with 2,2'-azobis(2-amidinopropane)hydrochloride (AAPH) or sodium hypochlorite (NaOCl). Oxidized CMs had a rapid phase of clearance, followed by a slow phase. Clearance of ox-CMs was decreased for corn oil but increased for linseed and fish oil particles. Differences in rats of uptake between CM types or treatment were independent of the rate of remnant formation, but were instead a consequence of decreased clearance. A greater triglyceride-to-cholesteryl ester ratio in liver suggested that there was less lipolysis of ox-CM triglyceride prior to uptake. Hepatic uptake of ox-CMs was decreased, whereas there was increased uptake in spleen. However, the uptake by Kupffer cells of ox-CMs was 43% of total liver uptake after AAPH treatment and 59% after NaOCl treatment, compared with 21% for control CMs. Collectively, our data show that oxidation can have differential effects on the rate of clearance of CMs and that ox-CMs are preferentially cleared by the reticuloendothelial system.

Topics: Amidines; Animals; Cholesterol Esters; Chylomicrons; Corn Oil; Dietary Fats, Unsaturated; Fish Oils; Kinetics; Kupffer Cells; Linseed Oil; Liver; Lymph; Oxidation-Reduction; Rats; Rats, Wistar; Sodium Hypochlorite; Spleen; Triglycerides

1995