sodium-dodecyl-sulfate and tryptophan-octyl-ester

Tryptophan octyl ester (TOE) represents an important model for membrane-bound tryptophan residues. In this article, we have explored the effect of sphere-to-rod transition of sodium dodecyl sulfate micelles on the dynamics of the membrane-bound tryptophan analogue, TOE, utilizing a combination of fluorescence spectroscopic approaches which include red edge excitation shift (REES). Our results show that REES and fluorescence spectroscopic parameters such as lifetime, anisotropy and acrylamide quenching of micelle-bound TOE are sensitive to the change in micellar organization accompanied by the sphere-to-rod transition.

Topics: Acrylamide; Anisotropy; Cell Membrane; Membrane Proteins; Micelles; Models, Chemical; Sodium Dodecyl Sulfate; Spectrometry, Fluorescence; Tryptophan