sodium-dodecyl-sulfate and sulforaphane

The intestinal immune system is continuously exposed to massive amounts of nanoparticles derived from food. Whether nanoparticles from plants we eat daily have a role in maintaining intestinal immune homeostasis is poorly defined. Here, we present evidence supporting our hypothesis that edible nanoparticles regulate intestinal immune homeostasis by targeting dendritic cells (DCs). Using three mouse colitis models, our data show that orally given nanoparticles isolated from broccoli extracts protect mice against colitis. Broccoli-derived nanoparticle (BDN)-mediated activation of adenosine monophosphate-activated protein kinase (AMPK) in DCs plays a role in not only prevention of DC activation but also induction of tolerant DCs. Adoptively transferring DCs pre-pulsed with total BDN lipids, but not sulforaphane (SFN)-depleted BDN lipids, prevented DSS-induced colitis in C57BL/6 (B6) mice, supporting the role of BDN SFN in the induction of DC tolerance. Adoptively transferring AMPK

Topics: Administration, Oral; Adoptive Transfer; AMP-Activated Protein Kinases; Animals; Anti-Inflammatory Agents; Brassica; Colitis, Ulcerative; Dendritic Cells; Disease Models, Animal; Enzyme Activation; Gene Expression; Humans; Immune Tolerance; Isothiocyanates; Lipids; Mice; Mice, Inbred C57BL; Nanoparticles; Plant Extracts; Sodium Dodecyl Sulfate; Sulfoxides