sodium-dodecyl-sulfate and guanidine-isothiocyanate

sodium-dodecyl-sulfate has been researched along with guanidine-isothiocyanate* in 1 studies

Other Studies

1 other study(ies) available for sodium-dodecyl-sulfate and guanidine-isothiocyanate

Article	Year
[Comparison of different methods for isolating total RNA from bulblet of Fritillaria anhuiensis]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 2012, Volume: 37, Issue:6 To optimize a simple and effective method for total RNA extraction from bulblet of Fritillaria anhuiensis.. Four methods, i. e. guanidine isothiocyanate, bentonite, modified SDS/phenol and the RNAiso plus, were used to extract total RNA from bulblet of F. anhuiensis. Then the results of the extraction were compared and analyzed by electrophoresis detection and RT-PCR verification.. The total RNA extracted by bentonite method were clear and no dispersion, the integrity of the RNA was well, and there was no obvious contamination with DNA and other impurities, was suitable for RT-PCR test.. The bentonite method is quick, economic, and efficient for total RNA extraction from bulblet of F. anhuiensis. Topics: Bentonite; DNA, Complementary; Electrophoresis; Fritillaria; Guanidines; Isothiocyanates; Phenol; Plant Roots; Plants, Medicinal; Reverse Transcriptase Polymerase Chain Reaction; RNA, Plant; Sodium Dodecyl Sulfate; Time Factors	2012

Article

Year

[Comparison of different methods for isolating total RNA from bulblet of Fritillaria anhuiensis].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 2012, Volume: 37, Issue:6

To optimize a simple and effective method for total RNA extraction from bulblet of Fritillaria anhuiensis.. Four methods, i. e. guanidine isothiocyanate, bentonite, modified SDS/phenol and the RNAiso plus, were used to extract total RNA from bulblet of F. anhuiensis. Then the results of the extraction were compared and analyzed by electrophoresis detection and RT-PCR verification.. The total RNA extracted by bentonite method were clear and no dispersion, the integrity of the RNA was well, and there was no obvious contamination with DNA and other impurities, was suitable for RT-PCR test.. The bentonite method is quick, economic, and efficient for total RNA extraction from bulblet of F. anhuiensis.

Topics: Bentonite; DNA, Complementary; Electrophoresis; Fritillaria; Guanidines; Isothiocyanates; Phenol; Plant Roots; Plants, Medicinal; Reverse Transcriptase Polymerase Chain Reaction; RNA, Plant; Sodium Dodecyl Sulfate; Time Factors

2012