sodium-dodecyl-sulfate and galline

General morphology, physical and chemical stability of nuclei, and postfertilization behavior of spermatozoa from transgenic mice [TgN (Prml Gal) 223 Bri] containing nuclear avian protamine (galline) were compared to those in the spermatozoa of wild-type (Wild) mice. Galline to protamine I ratios in spermatozoal nuclei of transgenic mice, strains 3175 (T75) and 3177 (T77), were 1.94 and 5.62, respectively. Live T75 and T77 spermatozoa were indistinguishable in their gross morphology from Wild spermatozoa. However, unlike Wild and T75 spermatozoa, T77 spermatozoa were vulnerable to mechanical handling, as about 40% of heads and tails were separated after gentle pipetting in suspension. Motility of T77 spermatozoa was markedly inferior to that of T75 and Wild. Chromatin heterogeneity and instability of transgenic spermatozoal nuclei were evident by transmission electron microscopy, staining reaction to Giemsa, and, as apparent by both light microscopy and flow cytometry, reaction to SDS detergent. Wild and T75 spermatozoa fertilized 90% and 60% of zona-intact oocytes in vitro, respectively. T77 spermatozoa completely failed to fertilize and bound to zona surfaces very weakly, and none of them inserted their heads into the zona. Although inefficiently, T77 spermatozoa could fertilize zona-free oocytes in vitro, indicating some ability to undergo capacitation and spontaneous acrosome reaction in vitro. After microsurgical injection into oocytes, the rate of nuclear decondensation was the greatest in rooster spermatozoa, followed by T77, T75, and Wild spermatozoa.

Topics: Animals; Cell Nucleus; Chromatin; Drug Stability; Female; Fertilization in Vitro; Male; Mice; Mice, Transgenic; Microscopy, Electron; Oocytes; Protamines; Sodium Dodecyl Sulfate; Sperm Head; Sperm Motility; Sperm Tail; Spermatozoa; Zona Pellucida