sodium-dodecyl-sulfate and bufogenin

sodium-dodecyl-sulfate has been researched along with bufogenin* in 1 studies

Other Studies

1 other study(ies) available for sodium-dodecyl-sulfate and bufogenin

Article	Year
Microemulsion electrokinetic chromatographic determination of bufadienolides in toad venom and in traditional Chinese medicine. Analytical and bioanalytical chemistry, 2006, Volume: 384, Issue:5 A microemulsion electrokinetic chromatographic (MEEKC) method has been developed and validated for determination of resibufogenin and cinobufagin in toad venom and in traditional Chinese medicine prepared from the venom. The MEEKC method involved use of sodium dodecyl sulfate (SDS) as surfactant, heptane as organic solvent, and butan-1-ol as co-solvent. To improve the separation, the effect of temperature and running buffer pH were evaluated. The optimized conditions (heptane 0.81% (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w), and 10 mmol L(-1) sodium tetraborate buffer, pH 9.2, and 298 nm as the detection wavelength) enabled useful and repeatable separation of the analytes. Topics: Amphibian Venoms; Animals; Anura; Bufanolides; Capillary Electrochromatography; Emulsions; Hydrogen-Ion Concentration; Medicine, Chinese Traditional; Molecular Conformation; Pharmaceutical Preparations; Reproducibility of Results; Sensitivity and Specificity; Sodium Dodecyl Sulfate; Stereoisomerism; Temperature; Time Factors	2006

Article

Year

Microemulsion electrokinetic chromatographic determination of bufadienolides in toad venom and in traditional Chinese medicine.

Analytical and bioanalytical chemistry, 2006, Volume: 384, Issue:5

A microemulsion electrokinetic chromatographic (MEEKC) method has been developed and validated for determination of resibufogenin and cinobufagin in toad venom and in traditional Chinese medicine prepared from the venom. The MEEKC method involved use of sodium dodecyl sulfate (SDS) as surfactant, heptane as organic solvent, and butan-1-ol as co-solvent. To improve the separation, the effect of temperature and running buffer pH were evaluated. The optimized conditions (heptane 0.81% (w/w), SDS 3.31% (w/w), butan-1-ol 6.61% (w/w), and 10 mmol L(-1) sodium tetraborate buffer, pH 9.2, and 298 nm as the detection wavelength) enabled useful and repeatable separation of the analytes.

Topics: Amphibian Venoms; Animals; Anura; Bufanolides; Capillary Electrochromatography; Emulsions; Hydrogen-Ion Concentration; Medicine, Chinese Traditional; Molecular Conformation; Pharmaceutical Preparations; Reproducibility of Results; Sensitivity and Specificity; Sodium Dodecyl Sulfate; Stereoisomerism; Temperature; Time Factors

2006