sodium-dodecyl-sulfate and 4-4-difluoro-4-bora-3a-4a-diaza-s-indacene

Several glycosphingolipids were labeled with the fluorphore Bodipy-Fl and analyzed using capillary electrophoresis with laser-induced fluorescence detection. GM1-, LacCer-, and Cer-Bodipy-Fl were prepared through acylation using the N-hydroxysuccinimide ester of Bodipy-Fl. Several other glycosphingolipids including GT1a-, GD1a-, GM2-, GM3-, GD3-, and GlcCer-Bodipy-Fl were enzymatically synthesized. Micellar electrokinetic capillary chromatography with a TRIS/CHES/SDS/α-cyclodextrin buffer produced better separation than an established borate/deoxycholate/methyl-β-cyclodextrin buffer. The nine Bodipy-Fl-labeled glycosphingolipid standards were separated in under 5 min, theoretical plate counts were between 640,000 and 740,000, and the limit of detection was approximately 3 pM or 240 ymol analyte injected onto the capillary.

Topics: alpha-Cyclodextrins; beta-Cyclodextrins; Borates; Boron Compounds; Chromatography, Micellar Electrokinetic Capillary; Deoxycholic Acid; Electrophoresis, Capillary; Fluorescent Dyes; Glycosphingolipids; Limit of Detection; Sodium Dodecyl Sulfate; Taurine; Tromethamine