sodium-dodecyl-sulfate and 1-dodecyl-3-methylimidazolium

sodium-dodecyl-sulfate has been researched along with 1-dodecyl-3-methylimidazolium* in 2 studies

Other Studies

2 other study(ies) available for sodium-dodecyl-sulfate and 1-dodecyl-3-methylimidazolium

ArticleYear
1-Dodecyl-3-methylimidazolium chloride-assisted sample preparation method for efficient integral membrane proteome analysis.
    Analytical chemistry, 2014, Aug-05, Volume: 86, Issue:15

    Due to their extremely hydrophobic nature, the analysis of integral membrane proteins (IMPs) is of great challenge. Although various additives have been applied to improve the solubility of IMPs, they still suffer from low solubilization efficiency, incompatibility with trypsin digestion, or interference with MS detection. Herein, the systematic study on the effect of ionic liquid structure on membrane protein solubilization and trypsin biocompatibility was performed, based on which 1-dodecyl-3-methylimidazolium chloride (C12Im-Cl) was selected for the sample preparation of IMPs. Compared with other commonly used additives, such as sodium dodecyl sulfate (SDS), Rapigest, and methanol, C12Im-Cl showed the best performance. In addition, with a strong cation exchange trap column, it could be easily removed after trypsin digestion, which not only was beneficial to avoid protein precipitation during digestion but also had no adverse effect on LC-MS-based separation and detection. Such a C12Im-Cl-assisted sample preparation method was further applied to the membrane proteome analysis of rat brain. Compared with the SDS-assisted method, 1.4 and 3.5 times improvement on the identified IMP and hydrophobic peptide number were achieved (251 vs 178, and 982 vs 279). All these results demonstrated that the C12Im-Cl-assisted sample preparation method is of great promise to promote the large-scale membrane proteome profiling.

    Topics: Animals; Brain; Chromatography, Liquid; Imidazoles; Ionic Liquids; Male; Membrane Proteins; Microscopy, Electron, Scanning; Proteomics; Rats; Rats, Sprague-Dawley; Sodium Dodecyl Sulfate; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

2014
C12mimBr ionic liquid/SDS vesicle formation and use as template for the synthesis of hollow silica spheres.
    Langmuir : the ACS journal of surfaces and colloids, 2010, Jul-20, Volume: 26, Issue:14

    The phase behavior of an aqueous catanionic surfactant system, composed of a long-chain imidazolium ionic liquid 1-dodecyl-3-methylimidazolium bromide (C(12)mimBr) and sodium dodecyl sulfate (SDS), is described. The phase diagram of the catanionic system was determined by electrical conductivity measurements and the formation of vesicles in a birefringent L(alpha) phase characterized by transmission electron microscopy (TEM) and freeze-fracture transmission electron microscopy (FF-TEM). Rheological measurements were used to characterize the macroscopic properties of the birefringent L(alpha) phase. Both electrostatic and hydrophobic interactions contribute to the vesicle formation in the catanionic system. Compared to the DTAB/SDS aqueous solution, differences between the imidazolium and trimethylammonium headgroups geometric packing and charge density induce the different phase behavior in each system. Silica hollow spheres, with diameters 30-60 nm and a wall thickness of 8-10 nm, were prepared by using the vesicles as the templates. The hollow silica spheres were characterized by TEM, scanning electron microscopy (SEM), and nitrogen adsorption-desorption. The results suggest additional application for ionic liquid based vesicles to be used as templates for the synthesis of hollow inorganic materials.

    Topics: Imidazoles; Ionic Liquids; Microscopy, Electron, Transmission; Silicon Dioxide; Sodium Dodecyl Sulfate; Solutions; Water

2010
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