sodium-cyanoborohydride has been researched along with beta-aspartyl-phosphate* in 1 studies
1 other study(ies) available for sodium-cyanoborohydride and beta-aspartyl-phosphate
| Article | Year |
|---|---|
Formation of an aspartyl phosphate intermediate in the reactions of nucleoside phosphotransferase from carrots.
The nucleoside phosphotransferase from carrots forms N-phosphorylhydroxylamine when substrates are hydrolysed in the presence of hydroxylamine. Denaturation of the enzyme after short incubation with the substrates leads to a protein, in which, after reduction with [3H]NaCNBH3 and complete hydrolysis with 6 M HCl, labelled homoserine can be detected. The first experiment provides evidence for an activated phosphorylenzyme, the second experiment shows that the intermediate is an acyl phosphate formed by a nucleophilic attack of an aspartate beta-carboxylate group. Topics: Aspartic Acid; Borohydrides; Chromatography, Ion Exchange; Chromatography, Paper; Electrochemistry; Electrophoresis, Paper; Homoserine; Hydrogen-Ion Concentration; Hydrolysis; Hydroxylamine; Hydroxylamines; Oxidation-Reduction; Phosphotransferases; Plants; Substrate Specificity | 1986 |