sodium-acetate--anhydrous has been researched along with dipicolinic-acid* in 2 studies
2 other study(ies) available for sodium-acetate--anhydrous and dipicolinic-acid
| Article | Year |
|---|---|
Fluorescence lifetime and intensity of terbium-doped dipicolinic acid in water, HCl, and sodium acetate buffer solutions.
The lifetimes of the individual fluorescing lines from the terbium-doped dipicolinic acid (DPA) complex have been measured and reported, for the first time to our knowledge. These lifetimes have been measured as a function of terbium and dipicolinic acid concentration, solvent pH, and solvent composition for water, HCl, and sodium acetate buffer solutions. Fluorescence lifetimes over the range from 0.75 to 1.07 ms were measured. The maximum fluorescence was obtained for distilled water solutions. Topics: Buffers; Fluorescent Dyes; Half-Life; Hydrochloric Acid; Picolinic Acids; Sodium Acetate; Spectrometry, Fluorescence; Terbium; Water | 2009 |
A highly sensitive HPLC method for determination of nanomolar concentrations of dipicolinic acid, a characteristic constituent of bacterial endospores.
A high-performance liquid chromatographic method with indirect fluorescence detection has been developed for quantification of dipicolinic acid, a major constituent of bacterial endospores. After separation on a reversed-phase column, a post-column reagent of sodium acetate at 1 mol l(-1) with 50 micromol l(-1) terbium chloride was added for complexation of dipicolinic acid. Terbium monodipicolinate complexes formed were quantified by measuring the fluorescence emission maximum at 548 nm after excitation with UV light at 270 nm wavelength. Parameters of post-column complexation were optimized to achieve a detection limit of 0.5 nmol DPA l(-1), corresponding to about 10(3) Desulfosporosinus orientis endospores per ml. The method was applied to the analysis of spore contamination in tuna and for estimating the endospore numbers in marine sediments. Topics: Animals; Chromatography, High Pressure Liquid; Geologic Sediments; Peptococcaceae; Picolinic Acids; Sensitivity and Specificity; Sodium Acetate; Spores, Bacterial; Terbium; Tuna | 2007 |