sodium-acetate--anhydrous and brassinolide

To understand molecular processes in living plant cells, quantitative spectro-microscopic technologies are required. By combining fluorescence lifetime spectroscopy with confocal microscopy, we studied the subcellular properties and function of a GFP-tagged variant of the plasma membrane-bound brassinosteroid receptor BRI1 (BRI1-GFP) in living cells of Arabidopsis seedlings. Shortly after adding brassinolide, we observed BRI1-dependent cell-wall expansion, preceding cell elongation. In parallel, the fluorescence lifetime of BRI1-GFP decreased, indicating an alteration in the receptor's physico-chemical environment. The parameter modulating the fluorescence lifetime of BRI1-GFP was found to be BL-induced hyperpolarization of the plasma membrane. Furthermore, for induction of hyperpolarization and cell-wall expansion, activation of the plasma membrane P-ATPase was necessary. This activation required BRI1 kinase activity, and was mediated by BL-modulated interaction of BRI1 with the P-ATPase. Our results were used to develop a model suggesting that there is a fast BL-regulated signal response pathway within the plasma membrane that links BRI1 with P-ATPase for the regulation of cell-wall expansion.

Topics: 2,4-Dichlorophenoxyacetic Acid; Adenosine Triphosphatases; Arabidopsis; Arabidopsis Proteins; Brassinosteroids; Cell Membrane; Cell Wall; Cholestanols; Electrophysiology; Green Fluorescent Proteins; Membrane Potentials; Nicotiana; Phosphorylation; Plants, Genetically Modified; Protein Kinases; Recombinant Fusion Proteins; Seedlings; Sodium Acetate; Steroids, Heterocyclic