sodium-acetate--anhydrous and aniline

Dietzia natronolimnaea JQ-AN was isolated from industrial wastewater containing aniline. Under aerobic conditions, the JQ-AN strain degraded 87% of the aniline in a 300 mg L(-1) aniline solution after 120 h of shake flask incubation in a medium containing sodium acetate. This strain had an unusually high salinity tolerance in minimal medium (0-6% NaCl, w/v). The optimal pH for microbial growth and aniline biodegradation was pH 8.0. Two liters of simulated aniline wastewater was created in a reactor at pH 8.0 and 3% NaCl (w/v), and biodegradation of aniline was tested over 7 days at 30 °C. For the initial concentrations of 100, 300, and 500 mg L(-1), 100%, 80.5% and 72% of the aniline was degraded, respectively. Strain JQ-AN may use an ortho-cleavage pathway for dissimilation of the catechol intermediate.

Topics: Actinomycetales; Alkalies; Aniline Compounds; Base Sequence; Biodegradation, Environmental; Bioreactors; Hydrogen-Ion Concentration; Metabolic Networks and Pathways; Molecular Sequence Data; Phylogeny; RNA, Ribosomal, 16S; Sodium Acetate; Sodium Chloride

This study presents the application of a rapid, simple and inexpensive thin-layer chromatography (TLC) for the analysis of fructooligosaccharides (FOSs) as feed additives (prebiotics) in complicated biological samples with minimal pre-treatment. Prebiotics have been monitored in different parts of the intestinal tract (jejunum, ileum and colon) of monogastric animals to which commercially available dietetic products containing fructooligosaccharides Raftifeed IPX, Raftilose and polysaccharide maltodextrin have been added into the feed. Thereby it contributes to a clarification of fructooligosaccharides and polysaccharides transformation in the digestive system. Chromatographic separation has been studied on different chromatographic systems (stationary and mobile phases). Optimal separation of fructooligosaccharides in dietetic products as well as in the samples from intestinal tract of monogastric animals has been achieved on glass-backed precoated silica gel layers impregnated with sodium acetate. The layers were developed with butanol-ethanol-water (5:3:2, v/v) in a vertical trough glass chamber with mobile phase vapour saturation. The visualisation of FOSs on chromatograms was performed with mixture of diphenylamine-aniline-phosphoric acid in acetone as primary detection reagent. Coloured spots of FOSs (blue-pink spots) on chromatograms have also been detected by reflectance densitometry at wavelength lambda=370nm. Simultaneously, the concentration of acetic acid, which is one of FOSs fermentation product, was monitored in the intestinal contents from jejunum, ileum and colon by capillary isotachophoresis.

Topics: 1-Butanol; Acetic Acid; Acetone; Aniline Compounds; Animals; Chromatography, Thin Layer; Dietary Fiber; Diphenylamine; Electrophoresis, Capillary; Ethanol; Gastrointestinal Contents; Humans; Oligosaccharides; Phosphoric Acids; Silicon Dioxide; Sodium Acetate; Water