sirolimus has been researched along with coelenterazine* in 1 studies
1 other study(ies) available for sirolimus and coelenterazine
Article | Year |
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Luciferase-Specific Coelenterazine Analogues for Optical Cross Talk-Free Bioassays.
Spectral overlaps in fluorescence (FL) and bioluminescence (BL) commonly cause optical cross talks. The present protocol introduces five different lineages of coelenterazine (CTZ) analogues, which have selectivity to a specific luciferase, and thus cross talk-free. For example, some CTZ analogues with ethynyl or styryl groups display dramatically biased BL to specific luciferases and pH by modifying the functional groups at the C-2 and C-6 positions of the imidazopyridinne backbone of CTZ. The optical cross talk-free feature is exemplified with the multiplex system, which simultaneously illuminated antiestrogenic and rapamycin activities without optical cross talks. This unique protocol contributes to specific and high-throughput BL imaging of multiple optical readouts in mammalian cells without optical contamination. Topics: Alkynes; Animals; Chlorocebus aethiops; COS Cells; Estrogen Receptor Modulators; Imidazoles; Immunosuppressive Agents; Luciferases; Luminescent Agents; Luminescent Measurements; Optical Imaging; Pyrazines; Sirolimus; Styrenes | 2021 |