sirolimus and benzamide

sirolimus has been researched along with benzamide* in 1 studies

Other Studies

1 other study(ies) available for sirolimus and benzamide

ArticleYear
Lipophilic benzamide and anilide derivatives as high-performance liquid chromatography internal standards: application to sirolimus (rapamycin) determination.
    Journal of chromatography. B, Biomedical sciences and applications, 1997, Dec-05, Volume: 703, Issue:1-2

    Finding a suitable internal standard in reversed-phase high-performance liquid chromatography is often difficult. A general approach for selecting and synthesizing the proper internal standard is presented and applied to a validated method for quantitation of sirolimus in several biological matrices. A series of fifteen N-alkylbenzamides, N-alkyltoluamides and N-alkanoylanilines with a log P range of 3.51 to 6.68 were synthesized as internal standards; N-undecyl-o-toluamide was evaluated most extensively. Sirolimus quantitation involves a simple sample clean-up procedure followed by isocratic chromatography on a heated C18 analytical column with an 70% methanol-water mobile phase and ultraviolet detection at 278 nm. This method was linear from 2.5 to 200 ng with a limit of quantitation of 2.5 ng using a 1-ml blood sample. Sirolimus recovery was above 72.1%. The intra-day and inter-day coefficients of variation were less than 11.7%. Several drugs and sirolimus metabolites do not interfere with the analysis. This method was used to measure sirolimus in blood from rats, rabbits and humans.

    Topics: Administration, Oral; Anilides; Animals; Benzamides; Chromatography, High Pressure Liquid; Circadian Rhythm; Humans; Immunosuppressive Agents; Injections, Intravenous; Linear Models; Polyenes; Rabbits; Rats; Reference Standards; Reproducibility of Results; Sirolimus

1997