sirolimus and 6-bromoindirubin-3--oxime

sirolimus has been researched along with 6-bromoindirubin-3--oxime* in 2 studies

Other Studies

2 other study(ies) available for sirolimus and 6-bromoindirubin-3--oxime

Article	Year
6-Bromoindirubin-3'-oxime (6BIO) prevents myocardium from aging by inducing autophagy. Aging, 2020, 12-26, Volume: 12, Issue:24 Topics: Aging; Animals; Antioxidants; Autophagy; Beclin-1; beta-Galactosidase; Cell Line; Cyclin-Dependent Kinase Inhibitor p16; Fibrosis; Glutathione; Heart; Indoles; Lipid Peroxidation; Mice; Myoblasts, Cardiac; Myocardium; Oxidative Stress; Oximes; Proto-Oncogene Proteins c-myc; Rats; Reactive Oxygen Species; Sirolimus; Superoxide Dismutase; Tumor Suppressor Protein p53	2020
Small molecules affect human dental pulp stem cell properties via multiple signaling pathways. Stem cells and development, 2013, Sep-01, Volume: 22, Issue:17 One fundamental issue regarding stem cells for regenerative medicine is the maintenance of stem cell stemness. The purpose of the study was to test whether small molecules can enhance stem cell properties of mesenchymal stem cells (MSCs) derived from human dental pulp (hDPSCs), which have potential for multiple clinical applications. We identified the effects of small molecules (Pluripotin (SC1), 6-bromoindirubin-3-oxime and rapamycin) on the maintenance of hDPSC properties in vitro and the mechanisms involved in exerting the effects. Primary cultures of hDPSCs were exposed to optimal concentrations of these small molecules. Treated hDPSCs were analyzed for their proliferation, the expression levels of pluripotent and MSC markers, differentiation capacities, and intracellular signaling activations. We found that small molecule treatments decreased cell proliferation and increased the expression of STRO-1, NANOG, OCT4, and SOX2, while diminishing cell differentiation into odonto/osteogenic, adipogenic, and neurogenic lineages in vitro. These effects involved Ras-GAP-, ERK1/2-, and mTOR-signaling pathways, which may preserve the cell self-renewal capacity, while suppressing differentiation. We conclude that small molecules appear to enhance the immature state of hDPSCs in culture, which may be used as a strategy for adult stem cell maintenance and extend their capacity for regenerative applications. Topics: Adolescent; Antigens, Surface; Biomarkers; Cell Differentiation; Cell Lineage; Cell Proliferation; Dental Pulp; Extracellular Signal-Regulated MAP Kinases; Gene Expression; Homeodomain Proteins; Humans; Immunosuppressive Agents; Indoles; Mesenchymal Stem Cells; Nanog Homeobox Protein; Octamer Transcription Factor-3; Oximes; Pyrazoles; Pyrimidines; ras GTPase-Activating Proteins; Signal Transduction; Sirolimus; SOXB1 Transcription Factors; TOR Serine-Threonine Kinases; Young Adult	2013

Article

Year

6-Bromoindirubin-3'-oxime (6BIO) prevents myocardium from aging by inducing autophagy.

Aging, 2020, 12-26, Volume: 12, Issue:24

Topics: Aging; Animals; Antioxidants; Autophagy; Beclin-1; beta-Galactosidase; Cell Line; Cyclin-Dependent Kinase Inhibitor p16; Fibrosis; Glutathione; Heart; Indoles; Lipid Peroxidation; Mice; Myoblasts, Cardiac; Myocardium; Oxidative Stress; Oximes; Proto-Oncogene Proteins c-myc; Rats; Reactive Oxygen Species; Sirolimus; Superoxide Dismutase; Tumor Suppressor Protein p53

2020

Small molecules affect human dental pulp stem cell properties via multiple signaling pathways.

Stem cells and development, 2013, Sep-01, Volume: 22, Issue:17

One fundamental issue regarding stem cells for regenerative medicine is the maintenance of stem cell stemness. The purpose of the study was to test whether small molecules can enhance stem cell properties of mesenchymal stem cells (MSCs) derived from human dental pulp (hDPSCs), which have potential for multiple clinical applications. We identified the effects of small molecules (Pluripotin (SC1), 6-bromoindirubin-3-oxime and rapamycin) on the maintenance of hDPSC properties in vitro and the mechanisms involved in exerting the effects. Primary cultures of hDPSCs were exposed to optimal concentrations of these small molecules. Treated hDPSCs were analyzed for their proliferation, the expression levels of pluripotent and MSC markers, differentiation capacities, and intracellular signaling activations. We found that small molecule treatments decreased cell proliferation and increased the expression of STRO-1, NANOG, OCT4, and SOX2, while diminishing cell differentiation into odonto/osteogenic, adipogenic, and neurogenic lineages in vitro. These effects involved Ras-GAP-, ERK1/2-, and mTOR-signaling pathways, which may preserve the cell self-renewal capacity, while suppressing differentiation. We conclude that small molecules appear to enhance the immature state of hDPSCs in culture, which may be used as a strategy for adult stem cell maintenance and extend their capacity for regenerative applications.

Topics: Adolescent; Antigens, Surface; Biomarkers; Cell Differentiation; Cell Lineage; Cell Proliferation; Dental Pulp; Extracellular Signal-Regulated MAP Kinases; Gene Expression; Homeodomain Proteins; Humans; Immunosuppressive Agents; Indoles; Mesenchymal Stem Cells; Nanog Homeobox Protein; Octamer Transcription Factor-3; Oximes; Pyrazoles; Pyrimidines; ras GTPase-Activating Proteins; Signal Transduction; Sirolimus; SOXB1 Transcription Factors; TOR Serine-Threonine Kinases; Young Adult

2013