sincalide and androsta-1-4-6-triene-3-17-dione

Microinjections of sulphated cholecystokinin octapeptide (sCCK-8) into the ventromedial nucleus of the hypothalamus inhibit lordosis behavior in receptive female rats. This effect of sCCK-8 seems to differentiate under the control of gonadal steroids shortly after birth. Neonatally castrated males and normal females show similar responses, while androgenized females are less sensitive to sCCK-8. The current study investigated estrogen's role on the differentiation of the response to sCCK-8. On the day of birth male rat pups were castrated, given sham surgeries, or implanted with the antiestrogen tamoxifen or the aromatase inhibitor androst-1, 4, 6-triene-3, 17-dione (ATD). Females were implanted with testosterone propionate or tamoxifen, or given sham surgeries. Implants were removed 10 days later. As adults, rats were tested for female sexual behavior after microinjections of sCCK-8 into the ventromedial nucleus of the hypothalamus. Neonatally castrated males, ATD-treated males, and control females showed profound inhibition of lordosis behavior after sCCK-8. These results suggest that elimination of estrogen postnatally prevents defeminization of the reproductive circuitry that responds to sCCK-8.

Topics: Androstatrienes; Animals; Animals, Newborn; Estradiol; Estrogens; Female; Male; Pregnancy; Rats; Sex Differentiation; Sexual Behavior, Animal; Sexual Maturation; Sincalide; Tamoxifen; Ventromedial Hypothalamic Nucleus