sinapyl-alcohol has been researched along with 5-hydroxyferulic-acid* in 1 studies
1 other study(ies) available for sinapyl-alcohol and 5-hydroxyferulic-acid
| Article | Year |
|---|---|
Enzymatic activities for lignin monomer intermediates highlight the biosynthetic pathway of syringyl monomers in Robinia pseudoacacia.
Most of the known 4-coumarate:coenzyme A ligase (4CL) isoforms lack CoA-ligation activity for sinapic acid. Therefore, there is some doubt as to whether sinapic acid contributes to sinapyl alcohol biosynthesis. In this study, we characterized the enzyme activity of a protein mixture extracted from the developing xylem of Robinia pseudoacacia. The crude protein mixture contained at least two 4CLs with sinapic acid 4-CoA ligation activity. The crude enzyme preparation displayed negligible sinapaldehyde dehydrogenase activity, but showed ferulic acid 5-hydroxylation activity and 5-hydroxyferulic acid O-methyltransferase activity; these activities were retained in the presence of competitive substrates (coniferaldehyde and 5-hydroxyconiferaldehyde, respectively). 5-Hydroxyferulic acid and sinapic acid accumulated in the developing xylem of R. pseudoacacia, suggesting, in part at least, sinapic acid is a sinapyl alcohol precursor in this species. Topics: Biosynthetic Pathways; Coenzyme A Ligases; Coumaric Acids; Hydroxylation; Lignin; Methylation; Methyltransferases; Phenylpropionates; Plant Proteins; Robinia; Xylem | 2017 |