silvestrol and betulonic-acid

Overexpression of murine double minute 2 (MDM2) results in the inactivation of p53 and causes cancer which is a leading cause of death in recent era. In recent decades, much attention has been paid to discover potential inhibitors against MDM2 in order to cure cancer. Outcomes from studies proposes that the MDM2 is a hot target to screen potent antagonists. Thus, this study aims at discovering natural compounds using several computational approaches to inhibit the MDM2 and to eliminate p53-MDM2 interaction, which would result in the reactivation of p53 activity. A library of 500 terpenes was prepared and several virtual screening approaches were employed to find out the best hits which could serve as p53-MDM2 antagonists. On the basis of the designed protocol, three terpenes were selected. In the present study, for the stability and validation of selected three protein-ligand complexes 20 ns molecular dynamics simulations and principal component analyses (PCA) were performed. Results found that the selected terpenes hits (3-

Topics: Computer Simulation; Drug Screening Assays, Antitumor; Enzyme Inhibitors; Gene Expression Regulation, Neoplastic; Humans; Molecular Dynamics Simulation; Neoplasms; Oleanolic Acid; Principal Component Analysis; Proto-Oncogene Proteins c-mdm2; Structure-Activity Relationship; Terpenes; Triterpenes; Up-Regulation