silicon and didodecyldimethylammonium

Whether driven by external mechanical stresses (shear flow) or induced by membrane-active peptides and/or proteins, the collective growth of tubules in membranous fluids has seldom been reported. The pearling destabilization of these membranous tubules which requires an activation of the shape distortion, often induced by optical tweezers, membrane-active biomolecules or an electrical field, has also rarely been observed under mild experimental conditions. Here we report such events of collective tubulation and pearling destabilization in sessile drops of a didodecyl-dimethylammonium bromide (DDAB) vesicular solution that are confined by a surrounding oil medium. Based on the wetting dynamics and the features of the tubulation process, we show that the growth of the tubules here relies on a mechanism of "pinning-induced pulling" from the retracting drop, rather than the classical hydrodynamic fingering instability. We show that the whole tubulation process is driven by a strong coupling between the bulk properties of the ternary (DAAB/water/oil) system and the dynamics of wetting. Finally, we discuss the pearling destabilization of these tubules under vanishing static interface tension and quite mild tensile force arising from their pulling. We show that under those mild conditions, shape disturbances readily grow, either as pearling waves moving toward the drop-reservoir or as Rayleigh-type peristaltic modulations. Besides revealing singular non-Rayleigh pearling modes, this work also brings new insights into the flow dynamics in membranous tubules anchored to an infinite reservoir.

Topics: Alkanes; Chemical Phenomena; Kinetics; Mechanical Phenomena; Microscopy, Video; Models, Chemical; Oils; Quaternary Ammonium Compounds; Rheology; Silicon; Solutions; Squalene; Surface Tension; Tensile Strength; Viscosity; Wetting Agents

Cationic amphiphile DDAB (dimethyl-dioctadecyl-ammonium-bromide) can spontaneously form water-dispersed and solid supported mimicking biomembrane structures as well as valuable DNA delivery vehicles whose shape, stability and transfection efficiency can be easily optimized on varying temperature, water content and chemical composition. In this framework, disclosing the thermotropic behavior of DDAB assemblies can be considered as an essential step in conceiving and developing new non-viral vector systems. Our work has been focused primarily on understanding the mesophase structure of silicon supported DDAB thin film on varying temperature at constant relative humidity by energy dispersive X-ray diffraction (EDXD). Diffraction results have then been employed in providing a more comprehensive dynamic light scattering (DLS) analysis of corresponding thermotropic water dispersed vesicles made up of DDAB alone and in combination with helper lecithin DOPC (1,2-dioleoyl-sn-glycero-3-phosphatidylcholine) liposomes. We found that above 55 °C silicon-supported DDAB films undergo a significant thinning effect, whilst DDAB-water vesicles exhibit a reduction in size polydispersity. Upon cooling to 25 °C a distinct silicon supported DDAB mesophase, exhibiting a relative humidity-dependent spacing, has been pointed out, and modeled in terms of a lyotropic metastable gel-crystalline phase.DDAB/DOPC-water vesicles show a temperature-dependent switching in size distribution, leading to promising biomedical applications.

Topics: Humidity; Light; Membranes, Artificial; Molecular Structure; Phosphatidylcholines; Quaternary Ammonium Compounds; Scattering, Radiation; Silicon; Surface-Active Agents; Temperature; Water; X-Ray Diffraction