sepharose has been researched along with potassium-phosphate* in 2 studies
2 other study(ies) available for sepharose and potassium-phosphate
| Article | Year |
|---|---|
A simple and rapid method for purifying staphylococcal exfoliative toxin A.
A rapid and efficient method of purification of staphylococcal exfoliative toxin A, the causative agent of staphylococcal scalded skin syndrome (SSSS), has been developed. It is based on ammonium sulfate precipitation of the culture supernatant and hydrophobic interaction chromatography on Phenyl-Sepharose CL-4B. This procedure results in 87-fold purification of this toxin, which appears as a single band in sodium dodecyl sulfate-polyacrylamide gels. Topics: Ammonium Sulfate; Animals; Buffers; Electrophoresis, Polyacrylamide Gel; Enterotoxins; Exfoliatins; Exfoliation Syndrome; Hydrogen-Ion Concentration; Mice; Mice, Inbred ICR; Phosphates; Potassium Compounds; Sepharose; Staphylococcus aureus; Time Factors | 2000 |
Protein adsorbents intended for use in aqueous two-phase systems.
Preparation of adsorbents with high partition coefficients in polyethylene glycol-dextran and polyethylene glycol-phosphate systems is described. These adsorbents may be used to carry to carry proteins away from the insoluble cell fragments generated during cell disruption. By chromatographic elution, proteins may be selectively desorbed in a reduced volume. Topics: Adsorption; Alcohol Dehydrogenase; Alcohol Oxidoreductases; Cell Fractionation; Dextrans; Gels; Hexokinase; Phosphates; Polyethylene Glycols; Potassium; Potassium Compounds; Protein Binding; Proteins; Saccharomyces cerevisiae; Sepharose; Solutions | 1984 |