sepharose and malic-acid

sepharose has been researched along with malic-acid* in 1 studies

Other Studies

1 other study(ies) available for sepharose and malic-acid

Article	Year
Purification of tumor mitochondrial malic enzyme by specific ligand affinity chromatography. Protein expression and purification, 1990, Volume: 1, Issue:2 A two-step chromatographic procedure, based on a specific ligand-binding approach, for the purification of tumor NAD(P)(+)-dependent malic enzyme is described. The enzyme was purified to near homogeneity by extraction from mitochondria, negative cellulose phosphate chromatography, ammonium sulfate precipitation, and application of specific elution from a malate-agarose column. The rationale for the use of the affinity column is also described. Topics: Ammonium Sulfate; Animals; Cellulose; Chemical Precipitation; Chromatography, Affinity; Liver Neoplasms, Experimental; Malate Dehydrogenase; Malates; Male; Mitochondria; Rats; Rats, Inbred Strains; Sepharose	1990

Article

Year

Purification of tumor mitochondrial malic enzyme by specific ligand affinity chromatography.

Protein expression and purification, 1990, Volume: 1, Issue:2

A two-step chromatographic procedure, based on a specific ligand-binding approach, for the purification of tumor NAD(P)(+)-dependent malic enzyme is described. The enzyme was purified to near homogeneity by extraction from mitochondria, negative cellulose phosphate chromatography, ammonium sulfate precipitation, and application of specific elution from a malate-agarose column. The rationale for the use of the affinity column is also described.

Topics: Ammonium Sulfate; Animals; Cellulose; Chemical Precipitation; Chromatography, Affinity; Liver Neoplasms, Experimental; Malate Dehydrogenase; Malates; Male; Mitochondria; Rats; Rats, Inbred Strains; Sepharose

1990