sepharose and maleimide

sepharose has been researched along with maleimide* in 1 studies

Other Studies

1 other study(ies) available for sepharose and maleimide

ArticleYear
Efficient Conjugation of Oligosaccharides to Polymer Particles through Furan/Maleimide Diels-Alder Reaction: Application to the Capture of Carbohydrate-Binding Proteins.
    Chembiochem : a European journal of chemical biology, 2017, Jan-17, Volume: 18, Issue:2

    Glycan-protein interactions play a crucial role in physiological and pathological events. Hence, improving the isolation of carbohydrate-binding proteins (i.e., lectins and anti-glycan antibodies) from complex media might not only lead to a better understanding of their function, but also provide solutions for public health issues, such as water contamination or the need for universal blood plasma. Here we report a rapid and efficient method for producing carbohydrate-based affinity adsorbents combining enzymatic synthesis and metal-free click chemistry. Both simple and complex glycans (maltose, blood group antigens A, B, and H) were readily modified by the addition of a furyl group at the reducing end without the need for protecting groups and were then efficiently conjugated to maleimide-activated Sepharose particles through Diels-Alder cycloaddition. These neoglycoconjugates showed high efficiency for the purification of lectins (concanavalin A and Ulex europaeus agglutinin), as well as for the capture of anti-A and anti-B blood group antibodies, opening new prospects for glycoproteomics and for the development of universal blood plasma.

    Topics: Click Chemistry; Concanavalin A; Cycloaddition Reaction; Fluorescein-5-isothiocyanate; Furans; Lectins; Maleimides; Microscopy, Fluorescence; Oligosaccharides; Polymers; Protein Binding; Receptors, Cell Surface; Sepharose; Spectrophotometry, Infrared

2017