sepharose and inositol-1-3-4-5-tetrakisphosphate

sepharose has been researched along with inositol-1-3-4-5-tetrakisphosphate* in 1 studies

Other Studies

1 other study(ies) available for sepharose and inositol-1-3-4-5-tetrakisphosphate

Article	Year
A purification strategy for inositol 1,4,5-trisphosphate 3-kinase from rat liver based upon heparin interaction chromatography. Cellular signalling, 1992, Volume: 4, Issue:3 Rat liver inositol 1,4,5-trisphosphate [Ins (1,4,5)P3] 3-kinase was purified in high yield by a three-step procedure reliant upon chromatography on heparin and calmodulin agarose. Purified enzyme was stable in the presence of the detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulphonate (CHAPS) (0.1-0.5%) and the sulphydryl reducing reagent dithiothreitol (DTT). The purified enzyme was activated 2-3-fold by Ca2+ (1 microM) in the presence of calmodulin. Pyrophosphate and heparin were identified as inhibitors of the enzyme. Topics: Animals; Binding, Competitive; Calcium; Calmodulin; Chromatography, Affinity; Heparin; Inositol Phosphates; Liver; Male; Phosphotransferases; Phosphotransferases (Alcohol Group Acceptor); Rats; Rats, Inbred Strains; Sepharose	1992

Article

Year

A purification strategy for inositol 1,4,5-trisphosphate 3-kinase from rat liver based upon heparin interaction chromatography.

Cellular signalling, 1992, Volume: 4, Issue:3

Rat liver inositol 1,4,5-trisphosphate [Ins (1,4,5)P3] 3-kinase was purified in high yield by a three-step procedure reliant upon chromatography on heparin and calmodulin agarose. Purified enzyme was stable in the presence of the detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulphonate (CHAPS) (0.1-0.5%) and the sulphydryl reducing reagent dithiothreitol (DTT). The purified enzyme was activated 2-3-fold by Ca2+ (1 microM) in the presence of calmodulin. Pyrophosphate and heparin were identified as inhibitors of the enzyme.

Topics: Animals; Binding, Competitive; Calcium; Calmodulin; Chromatography, Affinity; Heparin; Inositol Phosphates; Liver; Male; Phosphotransferases; Phosphotransferases (Alcohol Group Acceptor); Rats; Rats, Inbred Strains; Sepharose

1992