sepharose and formamide

sepharose has been researched along with formamide* in 2 studies

Other Studies

2 other study(ies) available for sepharose and formamide

Article	Year
Purification of genomic DNA extracted from environmental sources for use in a polymerase chain reaction. Cold Spring Harbor protocols, 2010, Volume: 2010, Issue:2 Topics: DNA; Formamides; Genetic Techniques; Polymerase Chain Reaction; Sepharose; Sodium Chloride	2010
A simple and efficient method for the separation and detection of small DNA fragments by electrophoresis in formamide containing agarose gels and Southern blotting to DBM-paper. Nucleic acids research, 1982, Oct-11, Volume: 10, Issue:19 We show in this report that DNA fragments smaller than 300 bp are separated with high resolution by electrophoresis in concentrated (up to 7%) agarose gels containing 50% formamide. The separated DNA fragments can subsequently be quantitatively transferred to DBM-paper [Alwine, J.C. et al., Proc. Natl. Acad. Sci. USA (1977) 74, 5350-54] using the Southern technique [Southern, E.M., J. Mol. Biol. (1975) 98, 503-517], while preserving the sharpness of the original gel pattern. Since thin (0.2-0.4 mm) and thick (up to 5 mm) agarose slab gels can be easily handled in vertical or horizontal apparatus, this method should prove to be a very useful extention of the Southern technique, applicable to a variety of analytical and preparative purposes. Topics: Base Composition; Diazonium Compounds; DNA; Electrophoresis, Agar Gel; Formamides; Indicators and Reagents; Molecular Weight; Nucleic Acid Hybridization; Oligodeoxyribonucleotides; Oligonucleotides; Sepharose	1982

Article

Year

Purification of genomic DNA extracted from environmental sources for use in a polymerase chain reaction.

Cold Spring Harbor protocols, 2010, Volume: 2010, Issue:2

Topics: DNA; Formamides; Genetic Techniques; Polymerase Chain Reaction; Sepharose; Sodium Chloride

2010

A simple and efficient method for the separation and detection of small DNA fragments by electrophoresis in formamide containing agarose gels and Southern blotting to DBM-paper.

Nucleic acids research, 1982, Oct-11, Volume: 10, Issue:19

We show in this report that DNA fragments smaller than 300 bp are separated with high resolution by electrophoresis in concentrated (up to 7%) agarose gels containing 50% formamide. The separated DNA fragments can subsequently be quantitatively transferred to DBM-paper [Alwine, J.C. et al., Proc. Natl. Acad. Sci. USA (1977) 74, 5350-54] using the Southern technique [Southern, E.M., J. Mol. Biol. (1975) 98, 503-517], while preserving the sharpness of the original gel pattern. Since thin (0.2-0.4 mm) and thick (up to 5 mm) agarose slab gels can be easily handled in vertical or horizontal apparatus, this method should prove to be a very useful extention of the Southern technique, applicable to a variety of analytical and preparative purposes.

Topics: Base Composition; Diazonium Compounds; DNA; Electrophoresis, Agar Gel; Formamides; Indicators and Reagents; Molecular Weight; Nucleic Acid Hybridization; Oligodeoxyribonucleotides; Oligonucleotides; Sepharose

1982