sepharose and 6-methyladenine

sepharose has been researched along with 6-methyladenine* in 1 studies

Other Studies

1 other study(ies) available for sepharose and 6-methyladenine

Article	Year
GATC-specific restriction and modification systems in treponemes. Letters in applied microbiology, 2004, Volume: 38, Issue:4 To investigate the presence of GATC-specific modification and restriction activities in rumen isolates of Treponema sp.. The presence of N6-methyladenine within GATC (Dam) sequences was analysed using isoschizomeric restriction endonucleases having different sensitivities to the methylation of the target sequence. A fast screening method was used for testing of site-specific endonuclease activities directly in crude cell extracts. Three out of six rumen isolates of Treponema sp. showed restriction activities. Restriction endonucleases were further purified by Heparin-Sepharose chromatography. Using PCR and specific primers, no sequence homologous to the T. pallidum dam gene was found.. Three rumen treponemal strains were documented to possess MboI isoschizomeric restriction-modification systems.. This is the first report on restriction activity in rumen treponemes. Topics: Adenine; Animals; Base Sequence; Chromatography, Affinity; Deoxyribonucleases, Type II Site-Specific; DNA Restriction-Modification Enzymes; Polymerase Chain Reaction; Rumen; Sepharose; Site-Specific DNA-Methyltransferase (Adenine-Specific); Substrate Specificity; Treponema	2004

Article

Year

GATC-specific restriction and modification systems in treponemes.

Letters in applied microbiology, 2004, Volume: 38, Issue:4

To investigate the presence of GATC-specific modification and restriction activities in rumen isolates of Treponema sp.. The presence of N6-methyladenine within GATC (Dam) sequences was analysed using isoschizomeric restriction endonucleases having different sensitivities to the methylation of the target sequence. A fast screening method was used for testing of site-specific endonuclease activities directly in crude cell extracts. Three out of six rumen isolates of Treponema sp. showed restriction activities. Restriction endonucleases were further purified by Heparin-Sepharose chromatography. Using PCR and specific primers, no sequence homologous to the T. pallidum dam gene was found.. Three rumen treponemal strains were documented to possess MboI isoschizomeric restriction-modification systems.. This is the first report on restriction activity in rumen treponemes.

Topics: Adenine; Animals; Base Sequence; Chromatography, Affinity; Deoxyribonucleases, Type II Site-Specific; DNA Restriction-Modification Enzymes; Polymerase Chain Reaction; Rumen; Sepharose; Site-Specific DNA-Methyltransferase (Adenine-Specific); Substrate Specificity; Treponema

2004